Construct: ORF TRCN0000480141
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018288.2_s317c1
- Derived from:
- ccsbBroadEn_13551
- DNA Barcode:
- CGGACAGCCAGTTTCAGTTCGCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RNF212 (285498)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480141
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 285498 | RNF212 | ring finger protein 212 | NM_001193318.3 | 96.6% | 95.7% | (many diffs) |
| 2 | human | 285498 | RNF212 | ring finger protein 212 | XM_017008041.1 | 49.1% | 36.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 909
- ORF length:
- 840
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggccaactgg gtgttctgta atcgctgctt ccagccgccc cacaggacgt 121 cgtgcttcag cctcaccaac tgcgggcacg tgtactgcga cgcctgcctc ggcaaaggta 181 aaaagaatga atgcttgatt tgtaaagctc cttgtcgtac agttttgctt tcaaagcata 241 ccgacgcaga tatccaggca ttcttcatga gcatagacag tctgtgtaag aagtactcca 301 gggaaacctc ccagtatctc aggctttccc gaggctgttg ccgtctcaag ttatgcccag 361 ccacatcctc caaggaggtg cccagaggct cgacccatgg aagccaagca gcagccagag 421 atccccaaga acactgggtc agcacaacac gggcacccag accaggatgc cggcggtccc 481 agagccagcc agaggcccat ggaaacacca tccaggacgc acctcacccc ctcaccctgc 541 TCCATCCCAG CAGGACCCTC ATTCACACCA AGTCCCCTTG GGGCCGGAAG TTGCTTGAGT 601 TCATAAAACA TGTCTGTTAT CACAGACATC AGAGCCACAG GCCGTGCGCT CCAGGGTGGT 661 TTTGCCAAGT GCTGCAGAGG CCAGGTGCTG TCTCAGGAGA GAAGACCCAG CAGACCCGGC 721 CAGACCCTCC AGCCACCTGT CTGCTCTGCC TCTCATGCCT CTCAGGCTTC CGGCATGGAC 781 CGTGGAGATG GCTGGCTCCA GCCTGGGCAG CACAGGCTCT GCCCAGTGAC CTCGTGGCCC 841 CACTGTTTGT CAGTTACACT GTGGAGGTGA GCGTCACAAA CGCAGGGTGG AGTTTTCCCG 901 CAGCTGTGTT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 961 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1021 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACGGACA GCCAGTTTCA GTTCGCGGAC 1081 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt