Construct: ORF TRCN0000480159

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006144.2_s317c1
Derived from:
ccsbBroadEn_07598
DNA Barcode:
AGATCCTCCGTTAGCACCAACTTT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PEMT (10400)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000480159
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10400 PEMT phosphatidylethanolamine N-... NM_148172.3 99.7% 99.1% 283G>A;634G>A
2 human 10400 PEMT phosphatidylethanolamine N-... NM_001267551.2 90.3% 89.8% 28_29ins66;217G>A;568G>A
3 human 10400 PEMT phosphatidylethanolamine N-... NM_001267552.2 89.7% 75.5% (many diffs)
4 human 10400 PEMT phosphatidylethanolamine N-... XM_006721418.4 88.9% 86.4% (many diffs)
5 human 10400 PEMT phosphatidylethanolamine N-... NM_007169.3 84% 83.4% 0_1ins111;172G>A;523G>A
6 human 10400 PEMT phosphatidylethanolamine N-... NM_148173.2 84% 83.4% 0_1ins111;172G>A;523G>A
7 human 10400 PEMT phosphatidylethanolamine N-... XM_024450532.1 84% 83.4% 0_1ins111;172G>A;523G>A
8 human 10400 PEMT phosphatidylethanolamine N-... XM_017024016.1 52.8% 52.5% 0_1ins333;301G>A
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
774
ORF length:
708
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgaa gagatctggg aacccgggag ccgaggtaac gaacagctcg gtggcagggc
121ctgactgctg cggaggcctc ggcaatattg attttagaca ggcagacttc tgcgttatga
181cccggctgct gggctacgtg gaccccctgg atcccagctt tgtggctgcc gtcatcacca
241tcaccttcaa tccgctctac tggaatgtgg ttgcacgatg ggaacacaag acccgcaagc
301tgagcagggc cttcggatcc ccctacctgg cctgctactc tctaagcatc accatcctgc
361tccTGAACTT CCTGCGCTCG CACTGCTTCA CGCAGGCCAT GCTGAGCCAG CCCAGGATGG
421AGAGCCTGGA CACCCCCGCG GCCTACAGCC TGGGCCTCGC GCTCCTGGGA CTGGGCGTCG
481TGCTCGTGCT CTCCAGCTTC TTTGCACTGG GGTTCGCTGG AACTTTCCTA GGTGATTACT
541TCGGGATCCT CAAGGAGGCG AGAGTGACCG TGTTCCCCTT CAACATCCTG GACAACCCCA
601TGTACTGGGG AAGCACAGCC AACTACCTGG GCTGGGCCAT CATGCACGCC AGCCCCACGG
661GCCTGCTCCT GACGGTGCTG GTGGCCCTCA CCTACATAAT GGCTCTCCTA TACGAAGAGC
721CCTTCACCGC TGAGATCTAC CGGCAGAAAG CCTCCGGGTC CCACAAGAGG AGCTGCCCAA
781CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG
841ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA
901TATCTTGTGG AAAGGACGAA GATCCTCCGT TAGCACCAAC TTTACGCGTT AAGTCgacaa
961tcaacctctg gattacaaaa tttgtgaaag att

Download FASTA (ORF) (Full)