Construct: ORF TRCN0000480159
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006144.2_s317c1
- Derived from:
- ccsbBroadEn_07598
- DNA Barcode:
- AGATCCTCCGTTAGCACCAACTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PEMT (10400)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480159
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10400 | PEMT | phosphatidylethanolamine N-... | NM_148172.3 | 99.7% | 99.1% | 283G>A;634G>A |
| 2 | human | 10400 | PEMT | phosphatidylethanolamine N-... | NM_001267551.2 | 90.3% | 89.8% | 28_29ins66;217G>A;568G>A |
| 3 | human | 10400 | PEMT | phosphatidylethanolamine N-... | NM_001267552.2 | 89.7% | 75.5% | (many diffs) |
| 4 | human | 10400 | PEMT | phosphatidylethanolamine N-... | XM_006721418.4 | 88.9% | 86.4% | (many diffs) |
| 5 | human | 10400 | PEMT | phosphatidylethanolamine N-... | NM_007169.3 | 84% | 83.4% | 0_1ins111;172G>A;523G>A |
| 6 | human | 10400 | PEMT | phosphatidylethanolamine N-... | NM_148173.2 | 84% | 83.4% | 0_1ins111;172G>A;523G>A |
| 7 | human | 10400 | PEMT | phosphatidylethanolamine N-... | XM_024450532.1 | 84% | 83.4% | 0_1ins111;172G>A;523G>A |
| 8 | human | 10400 | PEMT | phosphatidylethanolamine N-... | XM_017024016.1 | 52.8% | 52.5% | 0_1ins333;301G>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 774
- ORF length:
- 708
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gagatctggg aacccgggag ccgaggtaac gaacagctcg gtggcagggc 121 ctgactgctg cggaggcctc ggcaatattg attttagaca ggcagacttc tgcgttatga 181 cccggctgct gggctacgtg gaccccctgg atcccagctt tgtggctgcc gtcatcacca 241 tcaccttcaa tccgctctac tggaatgtgg ttgcacgatg ggaacacaag acccgcaagc 301 tgagcagggc cttcggatcc ccctacctgg cctgctactc tctaagcatc accatcctgc 361 tccTGAACTT CCTGCGCTCG CACTGCTTCA CGCAGGCCAT GCTGAGCCAG CCCAGGATGG 421 AGAGCCTGGA CACCCCCGCG GCCTACAGCC TGGGCCTCGC GCTCCTGGGA CTGGGCGTCG 481 TGCTCGTGCT CTCCAGCTTC TTTGCACTGG GGTTCGCTGG AACTTTCCTA GGTGATTACT 541 TCGGGATCCT CAAGGAGGCG AGAGTGACCG TGTTCCCCTT CAACATCCTG GACAACCCCA 601 TGTACTGGGG AAGCACAGCC AACTACCTGG GCTGGGCCAT CATGCACGCC AGCCCCACGG 661 GCCTGCTCCT GACGGTGCTG GTGGCCCTCA CCTACATAAT GGCTCTCCTA TACGAAGAGC 721 CCTTCACCGC TGAGATCTAC CGGCAGAAAG CCTCCGGGTC CCACAAGAGG AGCTGCCCAA 781 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 841 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 901 TATCTTGTGG AAAGGACGAA GATCCTCCGT TAGCACCAAC TTTACGCGTT AAGTCgacaa 961 tcaacctctg gattacaaaa tttgtgaaag att