Construct: ORF TRCN0000480160
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005602.1_s317c1
- Derived from:
- ccsbBroadEn_01493
- DNA Barcode:
- GCATGCGTTAGAAGCGTTCGTTGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SCO1 (6341)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480160
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6341 | SCO1 | SCO cytochrome c oxidase as... | NM_004589.4 | 100% | 100% | |
| 2 | mouse | 52892 | Sco1 | SCO1 cytochrome c oxidase a... | XM_006533721.1 | 54.7% | 53.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 969
- ORF length:
- 903
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gatgctggtc ctagtacccg gacgagttat gcggcctctg ggtggccaac 121 tttggcgctt cttgcctcgc ggactcgagt tttggggccc agccgagggg actgcgagag 181 tcttgctgag gcagttctgc gcgcggcaag cggaggcgtg gcgtgcctcg gggcgccctg 241 gctattgcct gggaacccgg cccctcagca ctgcgaggcc gccacccccg tggtcgcaga 301 agggccccgg agactccacg cgcccctcga agcccgggcc tgtttcctgg aagtctttag 361 caatcacatt tgctattgga ggagctttac tggctggaat gaagcacgtc aagaaagaaa 421 aggcagagaa gttagagaag gaacggcagc gacacatcgg caagccttta cttgggggac 481 cgttttccct cacaactcat actggggagc gtaaaactga caaggactac ttgggtcagt 541 ggttattgat ttattttggc ttcactcatt gcccTGATGT CTGTCCAGAA GAACTAGAAA 601 AGATGATTCA AGTCGTGGAT GAAATAGATA GCATTACAAC TCTGCCAGAT CTAACTCCAC 661 TTTTCATCAG CATTGACCCA GAGAGGGACA CAAAAGAAGC CATCGCAAAT TATGTGAAAG 721 AATTTTCTCC CAAACTGGTT GGCTTGACTG GCACGAGAGA AGAGGTCGAT CAAGTGGCCA 781 GAGCATACAG AGTGTATTAC AGCCCTGGCC CCAAGGACGA AGATGAAGAC TACATAGTGG 841 ATCACACAAT AATAATGTAC TTGATTGGAC CAGATGGTGA GTTTCTAGAT TATTTTGGCC 901 AGAACAAGAG GAAGGGAGAA ATAGCTGCTT CAATTGCCAC ACACATGAGG CCATACAGAA 961 AAAAGAGCTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1021 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1081 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGCATGC GTTAGAAGCG TTCGTTGAAC 1141 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt