Construct: ORF TRCN0000480162
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001544.1_s317c1
- Derived from:
- ccsbBroadEn_08649
- DNA Barcode:
- CCTGGACACAATCTCTGCAAATTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- JPH1 (56704)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480162
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56704 | JPH1 | junctophilin 1 | NM_001317830.2 | 99.8% | 100% | 9C>A;1242T>C |
| 2 | human | 56704 | JPH1 | junctophilin 1 | NM_001363050.1 | 99.8% | 100% | 9C>A;1242T>C |
| 3 | human | 56704 | JPH1 | junctophilin 1 | NM_001363051.1 | 99.8% | 100% | 9C>A;1242T>C |
| 4 | human | 56704 | JPH1 | junctophilin 1 | NM_020647.4 | 99.8% | 100% | 9C>A;1242T>C |
| 5 | mouse | 57339 | Jph1 | junctophilin 1 | NM_020604.2 | 89.6% | 93% | (many diffs) |
| 6 | mouse | 57339 | Jph1 | junctophilin 1 | XM_011238396.2 | 89.6% | 93% | (many diffs) |
| 7 | mouse | 57339 | Jph1 | junctophilin 1 | XM_011238397.2 | 89.6% | 93% | (many diffs) |
| 8 | mouse | 57339 | Jph1 | junctophilin 1 | XM_011238398.2 | 89.6% | 93% | (many diffs) |
| 9 | mouse | 57339 | Jph1 | junctophilin 1 | XM_011238399.2 | 89.6% | 93% | (many diffs) |
| 10 | mouse | 57339 | Jph1 | junctophilin 1 | XR_001785237.1 | 69.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2052
- ORF length:
- 1983
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gacgggagga aggttcgact tcgacgatgg cggcacctac tgcggcggct 121 gggaggaggg caaggcgcac gggcatggca tctgcacggg gcccaagggc cagggcgagt 181 actcgggctc ctggtcgcac ggcttcgagg tggtcggagg ctacacctgg cccagcggca 241 acacctacca gggctactgg gcgcagggca agcggcacgg gctgggggtg gagacgaagg 301 gcaagtggat gtaccggggg gagtggtcac atggtttcaa ggggcgctac ggggtccggc 361 agagcctgtg cacccccgct cgctacgagg gtacctggag taacgggctg caagacgggt 421 acggcgtgga gacctacggg gacggaggta cctaccaggg ccagtgggcc ggaggcatgc 481 ggcatggcta cggcgtgcgc cagagcgtgc cctacggcat ggccacggtg atccgctcac 541 cgctgcgtac ctcgctggcc tcgctgcgca gcgagcagag caatggcagc gtgctccacg 601 acgccgcagc cgccgccgac agcccggccg gcacccgcgg cggtttcgtg ctcaacttcc 661 acgcagacgc tgagctagcg ggcaagaaga agggcggcct cttccggagg ggctcccttc 721 ttggaagcat gaaacttcgc aagtccgaat ccaagtcttc catctcgagc aagcgcagct 781 ctgtccgcag cgacgcggcc atgagcagaa ttagttccag cgatgccaac tccacgatca 841 gctttggcga tgtagattgt gatttttgcc cggtggaaga ccacgtggac gccaccacca 901 cggaaaccta catgggcgag tggaagaacg acaagcgcaa cggcttcggc gttagcgagc 961 gctccaatgg catgaagtat gaaggggagt gggcaaataa caagaggcat ggatatggct 1021 gtaccgtgtt tcctgacggc tccaaagaag agggaaaata caaaaataat attctggtcc 1081 gtgggataag gaagcagctt ataccaataa gacatacaaa aactagggag aaggtggaca 1141 gagcaattga aggcgcccaa agggcagctg ccatggccag aaccaaagtg gaaatagcaa 1201 attcaaggac tgcacatgcc agagcgaagg ccgatgccgc cgaccaggcc gcgctggccg 1261 ctcgccagga gtgcgacatc gcgagagctg tggccaggga gctgtcaccc gatttctacc 1321 aaccaggccc tgattacgtc aaacagagat ttcaggaagg tgtagatgct aaagaaaatc 1381 cagaagaaaa ggtaccagaa aagccaccta caccaaagga gtctcctcat ttttatcgca 1441 aaggcacgac acccccaaga tctcctgagg caagtcccaa acacagccac tctcctgctt 1501 cctccccaaa gcccctgaag aagcaaaacc ccagctcagg ggcgagactc aaccaagaca 1561 aaaggagtgt ggctgatgag caggtgacgg ccattgtcaa taagcccttg atgtcaaagg 1621 ctcccacgaa ggaggcagga gcggttgtgc cccagtccaa gtactctggc cgccaccaca 1681 tccccaaccc cagtaacggG GAGCTGCATT CTCAGTATCA CGGCTACTAC GTGAAGCTGA 1741 ACGCCCCCCA GCACCCTCCA GTAGACGTGG AGGACGGCGA TGGATCCAGC CAGTCTTCCT 1801 CAGCACTGGT GCACAAGCCA TCCGCTAACA AGTGGAGTCC CTCCAAATCT GTGACAAAAC 1861 CAGTTGCCAA AGAAAGCAAA GCTGAGCCAA AAGCTAAGAA GTCTGAACTT GCTATACCAA 1921 AGAATCCAGC AAGCAACGAT TCATGCCCTG CTTTGGAAAA AGAAGCCAAT TCAGGCCCTA 1981 ATTCAATCAT GATTGTCCTT GTCATGCTGT TGAATATCGG GTTGGCCATT CTTTTTGTTC 2041 ACTTTCTAAC TTTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 2101 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 2161 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACCT GGACACAATC TCTGCAAATT 2221 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t