Construct: ORF TRCN0000480168
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018786.2_s317c1
- Derived from:
- ccsbBroadEn_15332
- DNA Barcode:
- GCTTTATGGCAATTGTACTATGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CLK3 (1198)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480168
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1198 | CLK3 | CDC like kinase 3 | NM_003992.4 | 11.3% | 11.2% | 1_1302del;1456C>T |
| 2 | human | 1198 | CLK3 | CDC like kinase 3 | NM_001130028.1 | 8.7% | 8.6% | 1_1746del;1900C>T |
| 3 | mouse | 102414 | Clk3 | CDC-like kinase 3 | XM_017313061.1 | 19% | 20% | (many diffs) |
| 4 | mouse | 102414 | Clk3 | CDC-like kinase 3 | XM_006510739.3 | 10.4% | 11% | (many diffs) |
| 5 | mouse | 102414 | Clk3 | CDC-like kinase 3 | XM_017313060.1 | 10.4% | 11% | (many diffs) |
| 6 | mouse | 102414 | Clk3 | CDC-like kinase 3 | NM_007713.4 | 8% | 8.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 216
- ORF end:
- 384
- ORF length:
- 168
- Sequence:
-
1 TTATGCGATG GAGTTTCCCC ACACTGAGTG GGTGGAGACT GAAGTTAGGC CAGCTTGGCA 61 CTTGATGTAA TTCTCCTTGG AATTTGCCCT TTTTGAGTTT GGATCTTGGT TCATTCTCAA 121 GCCTCAGACA GTGGTTCAAA GTTTTTTTCT TCCATTTCAG GTGTCGTGAG GCTAGCATCG 181 ATTGATCAAC AAGTTTGTAC AAAAAAGTTG GCACCATGCT CCAAGACTCC CTGGAGCACG 241 TGCAGCTGTT TGACCTGATG AGGAGGATGT TAGAATTTGA CCCTGCCCAG CGCATCACAC 301 TGGCCGAGGC CCTGCTGCAC CCCTTCTTTG CTGGCCTGAC CCCTGAGGAG CGGTCCTTCC 361 ACACCAGCTG CAACCCAAGC AGATTGCTAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 421 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 481 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAG CTTTATGGCA 541 ATTGTACTAT GTCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 601 att