Construct: ORF TRCN0000480188
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006316.1_s317c1
- Derived from:
- ccsbBroadEn_06258
- DNA Barcode:
- CGGTATCCGGCTTGTAACTCAATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GATA2 (2624)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480188
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2624 | GATA2 | GATA binding protein 2 | NM_001145661.2 | 99.9% | 100% | 15C>G |
| 2 | human | 2624 | GATA2 | GATA binding protein 2 | NM_032638.5 | 99.9% | 100% | 15C>G |
| 3 | human | 2624 | GATA2 | GATA binding protein 2 | NM_001145662.1 | 97% | 97% | 15C>G;1016_1017ins42 |
| 4 | mouse | 14461 | Gata2 | GATA binding protein 2 | NM_008090.5 | 90% | 97.9% | (many diffs) |
| 5 | mouse | 14461 | Gata2 | GATA binding protein 2 | XM_006505539.3 | 90% | 97.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1506
- ORF length:
- 1440
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggtggcgccg gagcagccgc gctggatggc gcacccggcc gtgctgaatg 121 cgcagcaccc cgactcacac cacccgggcc tggcgcacaa ctacatggaa cccgcgcagc 181 tgctgcctcc agacgaggtg gacgtcttct tcaatcacct cgactcgcag ggcaacccct 241 actatgccaa ccccgctcac gcgcgggcgc gcgtctccta cagccccgcg cacgcccgcc 301 tgaccggagg ccagatgtgc cgcccacact tgttgcacag cccgggtttg ccctggctgg 361 acgggggcaa agcagccctc tctgccgctg cggcccacca ccacaacccc tggaccgtga 421 gccccttctc caagacgcca ctgcacccct cagctgctgg aggccctgga ggcccactct 481 ctgtgtaccc aggggctggg ggtgggagcg ggggaggcag cgggagctca gtggcctccc 541 tcacccctac agcagcccac tctggctccc accttttcgg cttcccaccc acgccaccca 601 aagaagtgtc tcctgaccct agcaccacgg gggctgcgtc tccagcctca tcttccgcgg 661 ggggtagtgc agcccgagga gaggacaagg acggcgtcaa gtaccaggtg tcactgacgg 721 agagcatgaa gatggaaagt ggcagtcccc tgcgcccagg cctagctact atgggcaccc 781 agcctgctac acaccacccc atccccacct acccctccta tgtgccggcg gctgcccacg 841 actacagcag cggactcttc caccccggag gcttcctggg gggaccggcc tccagcttca 901 cccctaagca gcgcagcaag gctcgttcct gttcagaagg ccgggagtgt gtcaactgtg 961 gggccacagc cacccctctc tggcggcggg acggcaccgg ccactacctg tgcaatgcct 1021 gtggcctcta ccacaagatg aatgggcaga accgaccact catcaagccc aagcgaagac 1081 tgtcggccgc cagaagagcc ggcacctgtt gtgcaaattg tcagacgaca accaccaccT 1141 TATGGCGCCG AAACGCCAAC GGGGACCCTG TCTGCAACGC CTGTGGCCTC TACTACAAGC 1201 TGCACAATGT TAACAGGCCA CTGACCATGA AGAAGGAAGG GATCCAGACT CGGAACCGGA 1261 AGATGTCCAA CAAGTCCAAG AAGAGCAAGA AAGGGGCGGA GTGCTTCGAG GAGCTGTCAA 1321 AGTGCATGCA GGAGAAGTCA TCCCCCTTCA GTGCAGCTGC CCTGGCTGGA CACATGGCAC 1381 CTGTGGGCCA CCTCCCGCCC TTCAGCCACT CCGGACACAT CCTGCCCACT CCGACGCCCA 1441 TCCACCCCTC CTCCAGCCTC TCCTTCGGCC ACCCCCACCC GTCCAGCATG GTGACCGCCA 1501 TGGGCTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1561 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1621 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACGGTATCCG GCTTGTAACT CAATCACGCG 1681 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt