Construct: ORF TRCN0000480207
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013969.1_s317c1
- Derived from:
- ccsbBroadEn_06707
- DNA Barcode:
- ACTACTCATTGCCAGGTTCCCCAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PDE6B (5158)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480207
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5158 | PDE6B | phosphodiesterase 6B | NM_001145291.1 | 99.9% | 99.8% | 1962G>T |
| 2 | human | 5158 | PDE6B | phosphodiesterase 6B | NM_000283.3 | 99.8% | 99.7% | 1962G>T;2502_2504delAGT |
| 3 | human | 5158 | PDE6B | phosphodiesterase 6B | XM_011513475.2 | 93.5% | 92.9% | (many diffs) |
| 4 | human | 5158 | PDE6B | phosphodiesterase 6B | XM_011513474.3 | 91.9% | 91.8% | (many diffs) |
| 5 | human | 5158 | PDE6B | phosphodiesterase 6B | XM_011513473.3 | 86.4% | 85.8% | (many diffs) |
| 6 | human | 5158 | PDE6B | phosphodiesterase 6B | XM_011513476.3 | 84.5% | 84.4% | (many diffs) |
| 7 | human | 5158 | PDE6B | phosphodiesterase 6B | NM_001145292.1 | 67.1% | 67% | 0_1ins837;1125G>T;1665_1667delAGT |
| 8 | human | 5158 | PDE6B | phosphodiesterase 6B | XM_017008285.1 | 67.1% | 66.9% | (many diffs) |
| 9 | human | 5158 | PDE6B | phosphodiesterase 6B | XM_017008286.1 | 67.1% | 66.9% | (many diffs) |
| 10 | human | 5158 | PDE6B | phosphodiesterase 6B | NM_001350154.2 | 62.1% | 61.2% | (many diffs) |
| 11 | human | 5158 | PDE6B | phosphodiesterase 6B | XM_017008284.1 | 62.1% | 61.2% | (many diffs) |
| 12 | human | 5158 | PDE6B | phosphodiesterase 6B | XM_011513478.2 | 53.4% | 52.5% | (many diffs) |
| 13 | human | 5158 | PDE6B | phosphodiesterase 6B | NM_001350155.2 | 50.2% | 49.3% | (many diffs) |
| 14 | mouse | 18587 | Pde6b | phosphodiesterase 6B, cGMP,... | NM_008806.2 | 85.3% | 93.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2625
- ORF length:
- 2559
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag cctcagtgag gagcaggccc ggagctttct ggaccagaac cccgattttg 121 cccgccagta ctttgggaag aaactgagcc ctgagaatgt ggccgcggcc tgcgaggacg 181 ggtgcccgcc ggactgcgac agcctccggg acctctgcca ggtggaggag agcacggcgc 241 tgctggagct ggtgcaggat atgcaggaga gcatcaacat ggagcgcgtg gtcttcaagg 301 tcctgcggcg cctctgcacc ctcctgcagg ccgaccgctg cagcctcttc atgtaccgcc 361 agcgcaacgg cgtggccgag ctggccacca ggcttttcag cgtgcagccg gacagcgtcc 421 tggaggactg cctggtgccc cccgactccg agatcgtctt cccactggac atcggggtcg 481 tgggccacgt ggctcagacc aaaaagatgg tgaacgtcga ggacgtggcc gagtgccctc 541 acttcagctc atttgctgac gagctcactg actacaagac aaagaatatg ctggccacac 601 ccatcatgaa tggcaaagac gtcgtggcgg tgatcatggc agtgaacaag ctcaacggcc 661 cattcttcac cagcgaagac gaagatgtgt tcttgaagta cctgaatttt gccacgttgt 721 acctgaagat ctatcacctg agctacctcc acaactgcga gacgcgccgc ggccaggtgc 781 tgctgtggtc ggccaacaag gtgtttgagg agctgacgga catcgagagg cagttccaca 841 aggccttcta cacggtgcgg gcctacctca actgcgagcg gtactccgtg ggcctcctgg 901 acatgaccaa ggagaaggaa ttttttgacg tgtggtctgt gctgatggga gagtcccagc 961 cgtactcggg cccacgcacg cctgatggcc gggaaattgt cttctacaaa gtgatcgact 1021 acatcctcca cggcaaggag gagatcaagg tcattcccac accctcagcc gatcactggg 1081 ccctggccag cggccttcca agctacgtgg cagaaagcgg ctttatttgt aacatcatga 1141 atgcttccgc tgacgaaatg ttcaaatttc aggaaggggc cctggacgac tccgggtggc 1201 tcatcaagaa tgtgctgtcc atgcccatcg tcaacaagaa ggaggagatt gtgggagtcg 1261 ccacatttta caacaggaaa gacgggaagc cctttgacga acaggacgag gttctcatgg 1321 agtccctgac acagttcctg ggctggtcag tgatgaacac cgacacctac gacaagatga 1381 acaagctgga gaaccgcaag gacatcgcac aggacatggt cctttaccac gtgaagtgcg 1441 acagggacga gatccagctc atcctgccaa ccagagcgcg cctggggaag gagcctgctg 1501 actgcgatga ggacgagctg ggcgaaatcc tgaaggagga gctgccaggg cccaccacat 1561 ttgacatcta cgaattccac ttctctgacc tggagtgcac cgaactggac ctggtcaaat 1621 gtggcatcca gatgtactac gagctgggcg tggtccgaaa gttccagatc ccccaggagg 1681 tcctggtgcg gttcctgttc tccatcagca aagggtaccg gagaatcacc taccacaact 1741 ggcgccacgg cttcaacgtg gcccagacga tgttcacgct gctcatgacc ggcaaactga 1801 agagctacta cacggacctg gaggccttcg ccatggtgac agccggcctg tgccatgaca 1861 tcgaccaccg cggcaccaac aacctgtacc agatgaagtc ccagaacccc ttggctaagc 1921 tccacggctc ctcgattttg gagcggcacc acctggagtt tgggaagttc ctgctctcgg 1981 aggagaccct gaacatctac cagaacctga accggcggca gcacgatcac gtgatccacc 2041 tgatggacat cgccatcatc gccacggacc tggccctgta cttcaagaag agagcgatgt 2101 ttcagaagat cgtggatgag tccaagaact accaggacaa gaagagctgg gtggagtacc 2161 tgtccctgga gacgacccgg aaggagatcg tcatggccat gatgatgaca gccTGCGACC 2221 TGTCTGCCAT CACCAAGCCC TGGGAAGTCC AGAGCAAGGT CGCACTTCTC GTGGCTGCTG 2281 AGTTCTGGGA GCAAGGTGAC TTGGAAAGGA CAGTCTTGGA TCAGCAGCCC ATTCCTATGA 2341 TGGACCGGAA CAAGGCGGCC GAGCTCCCCA AGCTGCAAGT GGGCTTCATC GACTTCGTGT 2401 GCACATTCGT GTACAAGGAG TTCTCTCGTT TCCACGAAGA GATCCTGCCC ATGTTCGACC 2461 GACTGCAGAA CAATAGGAAA GAGTGGAAGG CGCTGGCTGA TGAGTATGAG GCCAAAGTGA 2521 AGGCTCTGGA GGAGAAGGAG GAGGAGGAGA GGGTGGCAGC CAAGAAAGGC ACAGAAATTT 2581 GCAATGGCGG CCCAGCACCC AAGTCTTCAA CCTGCTGTAT CCTGTGCCCA ACTTTCTTGT 2641 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 2701 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 2761 GAAAGGACGA ACTACTCATT GCCAGGTTCC CCACACGCGT TAAGTCgaca atcaacctct 2821 ggattacaaa atttgtgaaa gatt