Construct: ORF TRCN0000480248
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004186.1_s317c1
- Derived from:
- ccsbBroadEn_06119
- DNA Barcode:
- TTGTCTCGATCAATTTCTACATAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DPYSL3 (1809)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480248
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1809 | DPYSL3 | dihydropyrimidinase like 3 | NM_001197294.2 | 99.9% | 99.8% | 982G>A |
| 2 | human | 1809 | DPYSL3 | dihydropyrimidinase like 3 | NM_001387.3 | 82.6% | 81.8% | (many diffs) |
| 3 | human | 1809 | DPYSL3 | dihydropyrimidinase like 3 | XM_011537574.2 | 81.9% | 80.9% | (many diffs) |
| 4 | mouse | 22240 | Dpysl3 | dihydropyrimidinase-like 3 | NM_001291455.1 | 89.7% | 96.9% | (many diffs) |
| 5 | mouse | 22240 | Dpysl3 | dihydropyrimidinase-like 3 | NM_009468.5 | 75% | 80.5% | (many diffs) |
| 6 | mouse | 22240 | Dpysl3 | dihydropyrimidinase-like 3 | NM_001136086.2 | 74.1% | 80.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2118
- ORF length:
- 2052
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ctcgggccgg aggggctggg acagctccca cgaagacgat ctgcccgtgt 121 acctggccag gccgggcacc acggaccagg tcccgcggca gaaatacggc ggcatgttct 181 gcaacgtgga gggcgccttc gagagcaaga cgctggattt cgatgccctc agcgtggggc 241 agcggggcgc gaagactcct cggagcggcc agggcagcga ccgaggatcg gggagtcggc 301 ccgggatcga gggggacacc ccgcgcaggg gccaaggccg ggaagagagc agggagcccg 361 cgcccgcctc ccccgccccc gccggggtag agatccggag cgccaccggc aaagaggtgt 421 tgcagaacct cggccccaag gacaagagtg accgtctcct tatcaaggga ggcagaatcg 481 tcaatgatga tcagtccttt tatgctgata tttacatgga agatggctta ataaaacaaa 541 ttggagacaa tctgattgtt cctggaggag tgaagaccat tgaagccaat gggaagatgg 601 tgatccctgg aggcatcgat gtccatactc acttccagat gccatataag ggaatgacca 661 cagtagatga cttcttccaa gggacaaagg cggccttagc aggtggcacc accatgatca 721 ttgaccatgt ggtgcctgag cctgagtcca gcctgactga ggcctatgag aaatggagag 781 agtgggctga tgggaagagt tgctgtgact atgccctgca tgtggacatc acccactgga 841 atgacagcgt caagcaggaa gtgcagaacc tcatcaagga caaaggggtt aactccttca 901 tggtttatat ggcttataag gatttgtatc aagtatctaa cacagagctc tatgagatct 961 tcacctgcct gggagagctg ggggccattg ctcaagttca tgctgagaat ggggatatca 1021 ttgcccagga gcaaacccgc atgttgaaaa tggggataac tggcccagaa ggccatgtac 1081 tgagcaggcc agaagagctg gaagctgagg ctgtgttccg tgccatcacc attgccagcc 1141 aaaccaattg ccctctctac gtcacaaagg tcatgagcaa gagtgcagct gacctcatct 1201 cacaagccag gaaaaaagga aatgtagtct ttggtgagcc catcactgcc agcctcggca 1261 tagatggaac ccattattgg agcaagaact gggccaaggc ggctgcattt gtgacatccc 1321 cacccctgag ccctgaccca actactccgg actacatcaa ctccttgctg gccagcgggg 1381 atctgcagct atctgggagt gcccactgca ccttcagcac tgcccagaaa gcaattggga 1441 aggacaactt cacagccatt cctgagggca ccaatggtgt ggaggagcgg atgtctgtca 1501 tctgggacaa ggctgtggcc acagggaaaa tggacgaaaa ccagttcgtg gctgtgacaa 1561 gcacaaacgc tgccaagatc ttcaacctgt atccccgcaa gggaagaata tctgtgggtt 1621 ctgacagcga cctcgtcatc tgggatccag atgctgtgaa gatcgtctct gccaagaacc 1681 accagtctgc ggcagagtac aacatctttg aagggatGGA GCTGCGCGGG GCTCCTCTGG 1741 TTGTCATCTG CCAGGGCAAG ATCATGCTGG AAGATGGCAA CCTGCACGTG ACCCAGGGGG 1801 CTGGCCGCTT CATACCCTGC AGCCCGTTCT CCGACTATGT CTACAAGCGC ATTAAAGCAC 1861 GGAGGAAGAT GGCAGACCTG CATGCCGTCC CAAGGGGCAT GTACGATGGG CCTGTGTTTG 1921 ACCTGACCAC CACCCCCAAA GGTGGCACCC CCGCAGGCTC TGCTCGGGGC TCTCCTACTC 1981 GGCCGAACCC ACCTGTGAGG AATCTTCATC AGTCGGGATT TAGCCTGTCA GGCACCCAAG 2041 TGGATGAGGG GGTTCGCTCA GCCAGCAAGC GCATCGTGGC GCCCCCAGGC GGCCGTTCTA 2101 ATATCACATC TCTGAGTTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 2161 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 2221 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATTGTCTC GATCAATTTC 2281 TACATAAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt