Construct: ORF TRCN0000480251

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004008.1_s317c1
Derived from:
ccsbBroadEn_14084
DNA Barcode:
ACATACTTGGTATATCCCCCATGA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SIRT1 (23411)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000480251
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23411 SIRT1 sirtuin 1 NM_001142498.1 81.4% 81.4% 0_1ins309
2 human 23411 SIRT1 sirtuin 1 NM_001314049.1 79.6% 77.3% (many diffs)
3 human 23411 SIRT1 sirtuin 1 NM_012238.5 74.2% 74.2% 1_576del
4 mouse 93759 Sirt1 sirtuin 1 NM_001159589.2 70.1% 72.3% (many diffs)
5 mouse 93759 Sirt1 sirtuin 1 NM_019812.3 66.5% 68.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1731
ORF length:
1665
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgat tggcacagat cctcgaacaa ttcttaaaga tttattgccg gaaacaatac
121ctccacctga gttggatgat atgacactgt ggcagattgt tattaatatc ctttcagaac
181caccaaaaag gaaaaaaaga aaagatatta atacaattga agatgctgtg aaattactgc
241aagagtgcaa aaaaattata gttctaactg gagctggggt gtctgtttca tgtggaatac
301ctgacttcag gtcaagggat ggtatttatg ctcgccttgc tgtagacttc ccagatcttc
361cagatcctca agcgatgttt gatattgaat atttcagaaa agatccaaga ccattcttca
421agtttgcaaa ggaaatatat cctggacaat tccagccatc tctctgtcac aaattcatag
481ccttgtcaga taaggaagga aaactacttc gcaactatac ccagaacata gacacgctgg
541aacaggttgc gggaatccaa aggataattc agtgtcatgg ttcctttgca acagcatctt
601gcctgatttg taaatacaaa gttgactgtg aagctgtacg aggagatatt tttaatcagg
661tagttcctcg atgtcctagg tgcccagctg atgaaccgct tgctatcatg aaaccagaga
721ttgtgttttt tggtgaaaat ttaccagaac agtttcatag agccatgaag tatgacaaag
781atgaagttga cctcctcatt gttattgggt cttccctcaa agtaagacca gtagcactaa
841ttccaagttc cataccccat gaagtgcctc agatattaat taatagagaa cctttgcctc
901atctgcattt tgatgtagag cttcttggag actgtgatgt cataattaat gaattgtgtc
961ataggttagg tggtgaatat gccaaacttt gctgtaaccc tgtaaagctt tcagaaatta
1021ctgaaaaacc tccacgaaca caaaaagaat tggcttattt gtcagagttg ccacccacac
1081ctcttcatgt ttcagaagac tcaagttcac cagaaagaac ttcaccacca gattcttcag
1141tgattgtcac acttttagac caagcagcta agagtaatga tgatttagat gtgtctgaat
1201caaaaggttg tatggaagaa aaaccacagg aagtacaaac ttctaggaat gttgaaagta
1261ttgctgaaca gatggaaaat ccggatttga agaatgttgg ttctagtact ggggagaaaa
1321atgaaagaac ttcagtggct ggaacagtga gaaaatgctg gccTAATAGA GTGGCAAAGG
1381AGCAGATTAG TAGGCGGCTT GATGGTAATC AGTATCTGTT TTTGCCACCA AATCGTTACA
1441TTTTCCATGG CGCTGAGGTA TATTCAGACT CTGAAGATGA CGTCTTATCC TCTAGTTCTT
1501GTGGCAGTAA CAGTGATAGT GGGACATGCC AGAGTCCAAG TTTAGAAGAA CCCATGGAGG
1561ATGAAAGTGA AATTGAAGAA TTCTACAATG GCTTAGAAGA TGAGCCTGAT GTTCCAGAGA
1621GAGCTGGAGG AGCTGGATTT GGGACTGATG GAGATGATCA AGAGGCAATT AATGAAGCTA
1681TATCTGTGAA ACAGGAAGTA ACAGACATGA ACTATCCATC AAACAAATCA TACCCAACTT
1741TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
1801CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
1861CTTGTGGAAA GGACGAACAT ACTTGGTATA TCCCCCATGA ACGCGTTAAG TCgacaatca
1921acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)

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