Construct: ORF TRCN0000480253
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002758.1_s317c1
- Derived from:
- ccsbBroadEn_11556
- DNA Barcode:
- CCAGTGCGGGGAATGCCATATAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ALDH1L1 (10840)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480253
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10840 | ALDH1L1 | aldehyde dehydrogenase 1 fa... | XM_017005614.2 | 60.6% | 59.5% | (many diffs) |
| 2 | human | 10840 | ALDH1L1 | aldehyde dehydrogenase 1 fa... | XM_017005613.2 | 59.9% | 58.8% | (many diffs) |
| 3 | human | 10840 | ALDH1L1 | aldehyde dehydrogenase 1 fa... | NM_012190.4 | 55.9% | 54.6% | 1470_1620del;1667_2706del |
| 4 | human | 10840 | ALDH1L1 | aldehyde dehydrogenase 1 fa... | XM_006713481.3 | 55.9% | 54.6% | 1470_1620del;1667_2706del |
| 5 | human | 10840 | ALDH1L1 | aldehyde dehydrogenase 1 fa... | XM_011512355.1 | 55.9% | 54.6% | 1470_1620del;1667_2706del |
| 6 | human | 10840 | ALDH1L1 | aldehyde dehydrogenase 1 fa... | XM_024453325.1 | 55.9% | 54.6% | 1470_1620del;1667_2706del |
| 7 | human | 10840 | ALDH1L1 | aldehyde dehydrogenase 1 fa... | NM_001270364.1 | 55.3% | 54% | 1_30del;1500_1650del;1697_2736del |
| 8 | human | 10840 | ALDH1L1 | aldehyde dehydrogenase 1 fa... | NR_072979.2 | 53.8% | 1_134del;1650_2813del | |
| 9 | human | 10840 | ALDH1L1 | aldehyde dehydrogenase 1 fa... | NM_001270365.2 | 44.7% | 43.4% | 350_351ins303;1167_1317del;1364_2403del |
| 10 | mouse | 107747 | Aldh1l1 | aldehyde dehydrogenase 1 fa... | NM_027406.1 | 47.8% | 49.2% | (many diffs) |
| 11 | mouse | 107747 | Aldh1l1 | aldehyde dehydrogenase 1 fa... | XM_006505279.3 | 47.8% | 49.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1581
- ORF length:
- 1515
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gattgcagtg attggacaga gcctgtttgg ccaggaagtt tactgccacc 121 tgaggaagga gggccacgaa gtggtgggtg tgttcactgt tccagacaag gatggaaagg 181 ccgaccccct gggtctggaa gctgagaagg atggagtgcc ggtattcaag tactcccggt 241 ggcgtgcaaa aggacaggct ttgcctgatg tggtggcaaa ataccaggct ttgggggccg 301 agctcaacgt cctgcccttc tgcagccaat tcatccccat ggagataatc agtgcccccc 361 ggcatggctc catcatctat cacccgtcac tgctccctag gcaccgaggg gcctcggcca 421 tcaactggac cctcattcac ggagataaga aaggggggtt ttccatcttc tgggcggatg 481 atggtctgga caccggagac ctgctgctgc agaaggagtg tgaggtgctc ccggacgaca 541 ccgtgagcac gctgtacaac cgcttcctct tccctgaagg catcaaaggg atggtgcagg 601 ccgtgaggct gatcgctgag ggcaaagccc ccagactccc tcagcctgag gaaggagcca 661 cctatgaggg gattcagaag aaggagacag ccaagatcaa ctgggaccag ccggcagagg 721 ccattcacaa ctggatccgc gggaacgaca aggtgccggg agcctggaca gaggcctgtg 781 aacagaaact gacatttttc aactcaacgc tgaacacttc aggcctggtg cccgagggag 841 acgctttgcc catcccagga gcccatcggc caggggtggt caccaaagca ggactcatcc 901 tctttgggaa tgatgacaaa atgctgctgg tgaagaatat tcagctggag gatggcaaaa 961 tgatcctggc ctcgaacttc tttaaggggg cagccagcag tgtccttgag ctgacagagg 1021 cagagctggt tactgcggag gctgtgcgga gtgtttggca gcggatcctc cccaaagtcc 1081 tggaggttga agactccact gatttcttca agtcaggggc cgcgtctgtg gacgttgtga 1141 ggctggtgga ggaagtgaag gagctgtgtg atggcctgga gttagaaaat gaagatgtgt 1201 acatggcatc cacctttggg gacttcatCC AGCTGTTAGT GAGGAAGCTG CGAGGGGACG 1261 ATGAGGAGGG CGAGTGCAGC ATTGACTACG TGGAAATGGC AGTGAACAAG CGCACTGTCC 1321 GCATGCCCCA CCAGCTCTTC ATTGGGGGGG AGTTCGTGGA TGCCGAGGGC GCCAAGACCT 1381 CTGAGACCAT CAATCCCACC GATGGAAGTG TCATCTGCCA GGTATCCCTG GCCCAAGTCA 1441 CCGACGTCGA CAAGGCAGTG GCCGCAGCCA AGGATGCCTT TGAGAATGGA CGGTGGGGGA 1501 AGATCAGTGC GCGGGACCGG GGCCGGCTGA TGTACAGGGC TCCACCATCC CCATCAACCA 1561 GGCCAGACCC AACCGCAACC TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1621 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1681 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACCAG TGCGGGGAAT 1741 GCCATATAAT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt