Construct: ORF TRCN0000480267
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013813.1_s317c1
- Derived from:
- ccsbBroadEn_07295
- DNA Barcode:
- ACTTAAAATATATCTTCATAGTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TNFRSF14 (8764)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480267
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8764 | TNFRSF14 | TNF receptor superfamily me... | NM_003820.3 | 99.8% | 99.6% | 50A>G |
| 2 | human | 8764 | TNFRSF14 | TNF receptor superfamily me... | XM_006711019.3 | 69.4% | 63.8% | 50A>G;551_552ins104;591_592ins154 |
| 3 | human | 8764 | TNFRSF14 | TNF receptor superfamily me... | XM_017002720.1 | 68.6% | 66% | (many diffs) |
| 4 | human | 8764 | TNFRSF14 | TNF receptor superfamily me... | NM_001297605.1 | 64.8% | 64.6% | 50A>G;552_553ins297 |
| 5 | human | 8764 | TNFRSF14 | TNF receptor superfamily me... | XM_011542383.2 | 58.3% | 39% | (many diffs) |
| 6 | human | 8764 | TNFRSF14 | TNF receptor superfamily me... | XR_001737513.2 | 45% | (many diffs) | |
| 7 | human | 8764 | TNFRSF14 | TNF receptor superfamily me... | XR_001737512.2 | 43.9% | (many diffs) | |
| 8 | human | 8764 | TNFRSF14 | TNF receptor superfamily me... | XR_001737511.1 | 40.3% | (many diffs) | |
| 9 | human | 8764 | TNFRSF14 | TNF receptor superfamily me... | XR_426638.3 | 30% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 915
- ORF length:
- 849
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gcctcctgga gactgggggc ctcctccctg gagatccacc cccagaaccg 121 acgtcttgag gctggtgctg tatctcacct tcctgggagc cccctgctac gccccagctc 181 tgccgtcctg caaggaggac gagtacccag tgggctccga gtgctgcccc aagtgcagtc 241 caggttatcg tgtgaaggag gcctgcgggg agctgacggg cacagtgtgt gaaccctgcc 301 ctccaggcac ctacattgcc cacctcaatg gcctaagcaa gtgtctgcag tgccaaatgt 361 gtgacccagc catgggcctg cgcgcgagcc ggaactgctc caggacagag aacgccgtgt 421 gtggctgcag cccaggccac ttctgcatcg tccaggacgg ggaccactgc gccgcgtgcc 481 gcgcttacgc caccTCCAGC CCGGGCCAGA GGGTGCAGAA GGGAGGCACC GAGAGTCAGG 541 ACACCCTGTG TCAGAACTGC CCCCCGGGGA CCTTCTCTCC CAATGGGACC CTGGAGGAAT 601 GTCAGCACCA GACCAAGTGC AGCTGGCTGG TGACGAAGGC CGGAGCTGGG ACCAGCAGCT 661 CCCACTGGGT ATGGTGGTTT CTCTCAGGGA GCCTCGTCAT CGTCATTGTT TGCTCCACAG 721 TTGGCCTAAT CATATGTGTG AAAAGAAGAA AGCCAAGGGG TGATGTAGTC AAGGTGATCG 781 TCTCCGTCCA GCGGAAAAGA CAGGAGGCAG AAGGTGAGGC CACAGTCATT GAGGCCCTGC 841 AGGCCCCTCC GGACGTCACC ACGGTGGCCG TGGAGGAGAC AATACCCTCA TTCACGGGGA 901 GGAGCCCAAA CCACTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 961 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1021 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA ACTTAAAATA TATCTTCATA 1081 GTGCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt