Construct: ORF TRCN0000480274
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006584.1_s317c1
- Derived from:
- ccsbBroadEn_15361
- DNA Barcode:
- ATCGAGGCCAAGAGTAGCCAAATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ASPH (444)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480274
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 444 | ASPH | aspartate beta-hydroxylase | NM_001164756.1 | 100% | 100% | |
| 2 | human | 444 | ASPH | aspartate beta-hydroxylase | XM_024447159.1 | 83.6% | 83.2% | (many diffs) |
| 3 | human | 444 | ASPH | aspartate beta-hydroxylase | NM_001164755.2 | 57.3% | 42.1% | (many diffs) |
| 4 | human | 444 | ASPH | aspartate beta-hydroxylase | NM_001164754.1 | 50.5% | 41.6% | (many diffs) |
| 5 | human | 444 | ASPH | aspartate beta-hydroxylase | NM_032466.4 | 47.7% | 40.3% | (many diffs) |
| 6 | human | 444 | ASPH | aspartate beta-hydroxylase | XM_024447157.1 | 43% | 36.3% | (many diffs) |
| 7 | human | 444 | ASPH | aspartate beta-hydroxylase | XM_017013438.1 | 24.7% | 18.9% | (many diffs) |
| 8 | human | 444 | ASPH | aspartate beta-hydroxylase | XM_005251243.2 | 24.6% | 18.9% | (many diffs) |
| 9 | human | 444 | ASPH | aspartate beta-hydroxylase | XM_005251247.2 | 24.6% | 19.5% | (many diffs) |
| 10 | human | 444 | ASPH | aspartate beta-hydroxylase | XM_017013432.1 | 23.8% | 18.3% | (many diffs) |
| 11 | human | 444 | ASPH | aspartate beta-hydroxylase | XM_017013430.1 | 23.8% | 18.2% | (many diffs) |
| 12 | human | 444 | ASPH | aspartate beta-hydroxylase | XM_024447155.1 | 23.7% | 18.8% | (many diffs) |
| 13 | human | 444 | ASPH | aspartate beta-hydroxylase | XM_017013434.1 | 23.1% | 19.3% | (many diffs) |
| 14 | human | 444 | ASPH | aspartate beta-hydroxylase | XM_005251239.2 | 23.1% | 19.3% | (many diffs) |
| 15 | human | 444 | ASPH | aspartate beta-hydroxylase | XM_005251246.3 | 22.6% | 16.9% | (many diffs) |
| 16 | human | 444 | ASPH | aspartate beta-hydroxylase | XM_005251236.2 | 22.6% | 19.2% | (many diffs) |
| 17 | human | 444 | ASPH | aspartate beta-hydroxylase | XM_005251235.2 | 22.5% | 19.1% | (many diffs) |
| 18 | human | 444 | ASPH | aspartate beta-hydroxylase | XM_017013425.1 | 22.3% | 18.7% | (many diffs) |
| 19 | human | 444 | ASPH | aspartate beta-hydroxylase | XM_017013421.1 | 22.2% | 18.8% | (many diffs) |
| 20 | human | 444 | ASPH | aspartate beta-hydroxylase | XM_017013420.1 | 21.8% | 18.5% | (many diffs) |
| 21 | human | 444 | ASPH | aspartate beta-hydroxylase | XM_017013437.2 | 21.8% | 16.3% | (many diffs) |
| 22 | human | 444 | ASPH | aspartate beta-hydroxylase | XM_017013419.1 | 21.8% | 18.5% | (many diffs) |
| 23 | human | 444 | ASPH | aspartate beta-hydroxylase | XM_005251242.3 | 21.1% | 17.4% | (many diffs) |
| 24 | human | 444 | ASPH | aspartate beta-hydroxylase | XM_017013429.2 | 20.7% | 17.2% | (many diffs) |
| 25 | human | 444 | ASPH | aspartate beta-hydroxylase | NM_004318.4 | 20.6% | 17.2% | (many diffs) |
| 26 | human | 444 | ASPH | aspartate beta-hydroxylase | XM_017013428.2 | 20.4% | 16.8% | (many diffs) |
| 27 | human | 444 | ASPH | aspartate beta-hydroxylase | XM_017013424.2 | 20% | 16.6% | (many diffs) |
| 28 | human | 444 | ASPH | aspartate beta-hydroxylase | XM_017013422.2 | 20% | 16.6% | (many diffs) |
| 29 | mouse | 65973 | Asph | aspartate-beta-hydroxylase | NM_001177856.1 | 88% | 90% | (many diffs) |
| 30 | mouse | 65973 | Asph | aspartate-beta-hydroxylase | XM_017320345.1 | 77.5% | 80.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 675
- ORF length:
- 609
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ccagcgtaag aatgccaaga gcagcggcaa cagcagcagc agcggctccg 121 gcagcggtag cacgagtgcg ggcagcagca gccccggggc ccggagagag acaaagcatg 181 gaggacacaa gaatgggagg aaaggcggac tctcaggaac ttcattcttc acgtggttta 241 tggtgattgc attgctgggc gtctggacat ctgtagctgt cgtttGGTTT GATCTTGTTG 301 ACTATGAGGA AGTTCTAGCC AAAGCAAAGG ACTTCCGTTA TAACTTATCA GAGGTGCTTC 361 AAGGAAAACT AGGAATCTAT GATGCTGATG GTGATGGAGA TTTTGATGTG GATGATGCCA 421 AAGTTTTATT AGGCCTGACC AAAGATGGCA GTAATGAAAA TATTGATTCT CTTGAGGAAG 481 TCCTTAATAT TTTAGCAGAG GAAAGTTCAG ATTGGTTTTA TGGTTTCCTC TCATTTCTCT 541 ATGATATAAT GACTCCTTTT GAAATGCTAG AAGAAGAAGA AGAAGAAAGC GAAACCGCAG 601 ATGGTGTTGA TGGTACGTCA CAGAATGAAG GGGTTCAGGG AAAGACTTGT GTCATATTGG 661 ATTTACATAA CCAGTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 721 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 781 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA ATCGAGGCCA AGAGTAGCCA 841 AATTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt