Construct: ORF TRCN0000480304
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002996.1_s317c1
- Derived from:
- ccsbBroadEn_05474
- DNA Barcode:
- TAATTTTTACCGTGGCATATTACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TCEAL5 (340543)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480304
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 340543 | TCEAL5 | transcription elongation fa... | NM_001012979.3 | 100% | 100% | |
| 2 | human | 85012 | TCEAL3 | transcription elongation fa... | NM_001006933.1 | 90.3% | 88.3% | (many diffs) |
| 3 | human | 85012 | TCEAL3 | transcription elongation fa... | NM_032926.3 | 90.3% | 88.3% | (many diffs) |
| 4 | human | 158931 | TCEAL6 | transcription elongation fa... | NM_001367790.1 | 89.8% | 86.4% | (many diffs) |
| 5 | human | 158931 | TCEAL6 | transcription elongation fa... | NM_001006938.3 | 83.1% | 78.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 687
- ORF length:
- 618
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaaaagctc tacaaagaaa atgaaggaaa gccagagaat gaaagaaacc 121 tagaaagtga gggaaagcca gaggatgagg gaagtacaga agatgaagga aagtcagacg 181 aggaagaaaa gccggacatg gaggggaaga cagaatgcga gggaaagcga gaggatgagg 241 gagagccagg tgatgaggga caactggaag atgagggaaa ccaggaaaag cagggcaagt 301 cTGAAGGTGA GGACAAGCCA CAAAGTGAGG GCAAGCCAGC CTCCCAGGCC AAGCCAGAGA 361 GCCAGCCGCG GGCCGCCGAA AAGCGCCCGG CTGAAGATTA TGTGCCCCGG AAAGCAAAAA 421 GAAAAACCGA CAGGGGGACG GACGATTCCC CCAAGGACTC TCAGGAGGAC TTACAAGAAA 481 GGCATCTGAG CAGTGAGGAG ATGATGAGAG AATGTGGAGA TGTGTCAAGG GCTCAGGAGG 541 AGCTAAGGAA AAAACAGAAA ATGGGTGGTT TTCATTGGAT GCAAAGAGAT GTACAGGATC 601 CATTCGCCCC AAGGGGCCAA CGGGGTGTGA GGGGAGTGAG GGGCGGAGGT AGGGGCCAGA 661 AAGACTTAGA AGATGTCCCA TATGTTTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 721 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 781 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATAATTTTT 841 ACCGTGGCAT ATTACTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 901 aagatt