Construct: ORF TRCN0000480335
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003684.1_s317c1
- Derived from:
- ccsbBroadEn_01232
- DNA Barcode:
- TTAGTAATCGGACCCAGGCCCTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UBL3 (5412)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480335
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5412 | UBL3 | ubiquitin like 3 | NM_007106.4 | 100% | 100% | |
| 2 | mouse | 24109 | Ubl3 | ubiquitin-like 3 | NM_011908.2 | 88.3% | 99.1% | (many diffs) |
| 3 | mouse | 24109 | Ubl3 | ubiquitin-like 3 | XM_006504832.3 | 88.3% | 99.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 417
- ORF length:
- 351
- Sequence:
-
1 tcTTCCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGTC CAGTAATGTC CCGGCGGATA TGATAAATTT GCGCCTCATT TTGGTAAGCG 121 GAAAAACAAA AGAGTTCCTG TTTTCTCCTA ACGATTCTGC TTCTGACATT GCAAAGCATG 181 TATATGACAA TTGGCCAATG GACTGGGAAG AAGAGCAGGT CAGCAGTCCA AATATTCTAC 241 GACTTATTTA TCAAGGACGA TTTCTACATG GAAATGTCAC ATTAGGAGCA TTAAAACTTC 301 CTTTTGGCAA AACAACAGTG ATGCATTTGG TGGCCAGAGA GACATTACCA GAGCCAAACT 361 CTCAAGGTCA GAGGAATCGT GAGAAGACTG GAGAGAGTAA TTGTTGTGTA ATCCTGTACC 421 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 481 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 541 ATATATCTTG TGGAAAGGAC GATTAGTAAT CGGACCCAGG CCCTTAACGC GTTAAGTCga 601 caatcaacct ctggattaca aaatttgtga aagatt