Construct: ORF TRCN0000480337

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001337.1_s317c1
Derived from:
ccsbBroadEn_06891
DNA Barcode:
GACCTACGAGGTCAGACATACTCG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RPS2 (6187)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000480337
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6187 RPS2 ribosomal protein S2 NM_002952.4 99.7% 100% 261T>C;735T>A
2 human 256355 RPS2P32 ribosomal protein S2 pseudo... NR_026676.1 76.7% (many diffs)
3 mouse 16898 Rps2 ribosomal protein S2 NM_008503.5 87.4% 98.6% (many diffs)
4 mouse 677044 Gm10653 predicted gene 10653; ribso... NR_003965.2 56.7% (many diffs)
5 mouse 108167548 Gm45855 predicted gene 45855; 40S r... XR_001778644.1 49.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
945
ORF length:
879
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc ggatgacgcc ggtgcagcgg gggggcccgg gggccctggt ggccctggga
121tggggaaccg cggtggcttc cgcggaggtt tcggcagtgg catccggggc cggggtcgcg
181gccgtggacg gggccggggc cgaggccgcg gagctcgcgg aggcaaggcc gaggataagg
241agtggatgcc cgtcaccaag ttgggccgct tggtcaagga catgaagatc aagtccctgg
301aggagatcta tctcttctcc ctgcccatta aggaatcaga gatcattgat ttcttcctgg
361gggcctctct caaggatgag gttttgaaga ttatgccagt gcagaagcag acccgtgccg
421gccagcgcac caggttcaag gcatttgttg ctatcgggga ctacaatggc cacgtcggtc
481tgggtgttaa gtgctccaag gaggtggcca ccgccatccg tggggccatc atcctggcca
541agctctccat cgtccCCGTG CGCAGAGGCT ACTGGGGGAA CAAGATCGGC AAGCCCCACA
601CTGTCCCTTG CAAGGTGACA GGCCGCTGCG GCTCTGTGCT GGTACGCCTC ATCCCTGCAC
661CCAGGGGCAC TGGCATCGTC TCCGCACCTG TGCCTAAGAA GCTGCTCATG ATGGCTGGTA
721TCGATGACTG CTACACCTCA GCCCGGGGCT GCACTGCCAC CCTGGGCAAC TTCGCCAAGG
781CCACCTTTGA TGCCATTTCA AAGACCTACA GCTACCTGAC CCCCGACCTC TGGAAGGAGA
841CTGTATTCAC CAAGTCTCCC TATCAGGAGT TCACTGACCA CCTCGTCAAG ACCCACACCA
901GAGTCTCCGT GCAGCGGACT CAGGCTCCAG CTGTGGCTAC AACATACCCA ACTTTCTTGT
961ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT
1021AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG
1081GAAAGGACGA GACCTACGAG GTCAGACATA CTCGACGCGT TAAGTCgaca atcaacctct
1141ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)