Construct: ORF TRCN0000480343
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015451.1_s317c1
- Derived from:
- ccsbBroadEn_11413
- DNA Barcode:
- TATCAAATCCGCTGCTCACGTCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PIEZO1 (9780)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480343
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9780 | PIEZO1 | piezo type mechanosensitive... | NM_001142864.4 | 14.1% | 11.2% | (many diffs) |
| 2 | mouse | 234839 | Piezo1 | piezo-type mechanosensitive... | XM_011248372.1 | 22.7% | 18.3% | (many diffs) |
| 3 | mouse | 234839 | Piezo1 | piezo-type mechanosensitive... | XM_006530959.3 | 17.5% | 14.1% | (many diffs) |
| 4 | mouse | 234839 | Piezo1 | piezo-type mechanosensitive... | XM_006530956.2 | 12.6% | 10.3% | (many diffs) |
| 5 | mouse | 234839 | Piezo1 | piezo-type mechanosensitive... | XM_006530954.2 | 12.5% | 10.2% | (many diffs) |
| 6 | mouse | 234839 | Piezo1 | piezo-type mechanosensitive... | XM_006530953.2 | 12.5% | 10.2% | (many diffs) |
| 7 | mouse | 234839 | Piezo1 | piezo-type mechanosensitive... | NM_001037298.1 | 12.5% | 10.2% | (many diffs) |
| 8 | mouse | 234839 | Piezo1 | piezo-type mechanosensitive... | XM_006530955.2 | 12.5% | 10.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1185
- ORF length:
- 1119
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gatttgcccg ttttcttcta aaagttctat agttttagct ctgaagtttc 121 gctctttgat ccactttgag aaatacccgc agcccaaagg gcagaagaag aagaagatcg 181 tcaagtacgg catgggtggc ctcatcatcc tcttcctcat cgccatcatc tggttcccgc 241 tgctcttcat gtcgctggtg cgctccgtgg ttggggttgt caaccagccc atcgatgtca 301 ccgtcaccct caagctgggc ggctatgagc cgctgttcac catgagcgcc cagcagccgt 361 ccatcatccc cttcacggcc caggcctatg aggagctgtc ccggcagttt gacccccagc 421 cgctggccat gcagttcatc agccagtaca gccctgagga catcgtcacg gcgcagattg 481 agggcagctc cggggcgctg tggcgcatca gtccccccag ccgtgcccag atgaagcggg 541 agctctacaa cggcacggcc gacatcaccc tgcgcttcac ctggaacttc cagagggacc 601 tggcgaaggg aggcactgtg gagtatgcca acgagaagca catgctggcc ctggccccca 661 acagcactgc acggcggcag ctggccagcc tgctcgaggg cacctcggat cagtctgtgg 721 tcatcccCAA TCTCTTCCCC AAGTACATCC GTGCCCCCAA CGGGCCCGAA GCCAACCCTG 781 TGAAGCAGCT GCAGCCCAAT GAGGAGGCCG ACTACCTCGG CGTGCGTATC CAGCTGCGGA 841 GGGAGCAGGG TGCGGGGGCC ACCGGCTTCC TCGAATGGTG GGTCATCGAG CTGCAGGAGT 901 GCCGGACCGA CTGCAACCTG CTGCCCATGG TCATTTTCAG TGACAAGCAT CATGGGGCTG 961 TACGTGTCCA TCGTGCTGGT CATCGGCAAG TTCGTGCGCG GATTCTTCAG CGAGATCTCG 1021 CACTCCATTA TGTTCGAGGA GCTGCCGTGC GTGGACCGCA TCCTCAAGCT CTGCCAGGAC 1081 ATCTTCCTGG TGCGGGAGAC TCGGGAGCTG GAGCTGGAGG AGGAGTTGTA TGCCAAGCTC 1141 ATCTTCCTCT ACCGCTCACC GGAGACCATG ATCAAGTGGA CTCGTGCCCA ACTTTCTTGT 1201 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1261 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1321 GAAAGGACGA TATCAAATCC GCTGCTCACG TCTCACGCGT TAAGTCgaca atcaacctct 1381 ggattacaaa atttgtgaaa gatt