Construct: ORF TRCN0000480345
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013945.1_s317c1
- Derived from:
- ccsbBroadEn_13022
- DNA Barcode:
- TTAGGACTGACAGGTGTCCCCCAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LOC113386 (113386)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480345
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 374928 | ZNF773 | zinc finger protein 773 | NR_130705.2 | 19% | (many diffs) | |
| 2 | human | 105372481 | ERVK3-1 | endogenous retrovirus group... | NR_144445.1 | 15.5% | 1_309del;532_1424del | |
| 3 | human | 108903150 | ZNF8-ERVK3-1 | ZNF8-ERVK3-1 readthrough | NR_144447.1 | 4.7% | 1_3588del;3811_4687del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 223
- ORF end:
- 445
- ORF length:
- 222
- Sequence:
-
1 GGGAGGGGTT TTATGCGATG GAGTTTCCCC ACACTGAGTG GGTGGAGACT GAAGTTAGGC 61 CAGCTTGGCA CTTGATGTAA TTCTCCTTGG AATTTGCCCT TTTTGAGTTT GGATCTTGGT 121 TCATTCTCAA GCCTCAGACA GTGGTTCAAA GTTTTTTTCT TCCATTTCAG GTGTCGTGAG 181 GCTAGCATCG ATTGATCAAC AAGTTTGTAC AAAAAAGTTG GCATGAGCCA GCGGAATCTG 241 AATGCAAAGA CAGAACAAGG GCCGACCGGA GTCACAATGA CATCCAACCC CATAACATGG 301 GGACAGATCA AGAAAACGAC ACAAGAAGCT GAGAAACTAC TGGAGCGCCA GGGTCAGGCA 361 AAAACCCCTG ACTCCATGTT CTTGGCCATG CTAGCTGTAG TGTCCTGTGC GTCTATAGGA 421 TCGGGTGAAC CACCAACCGG CAATTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT 481 AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA 541 CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TTAGGACTGA 601 CAGGTGTCCC CCACACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa 661 gatt