Construct: ORF TRCN0000480366
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018230.1_s317c1
- Derived from:
- ccsbBroadEn_07840
- DNA Barcode:
- TAAGGCCCGTCGTGCCAAAAAAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ANKLE2 (23141)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480366
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23141 | ANKLE2 | ankyrin repeat and LEM doma... | NM_015114.3 | 99.8% | 99.4% | (many diffs) |
| 2 | human | 23141 | ANKLE2 | ankyrin repeat and LEM doma... | XM_005266159.3 | 93.2% | 92.8% | (many diffs) |
| 3 | human | 23141 | ANKLE2 | ankyrin repeat and LEM doma... | XM_005266160.2 | 93.2% | 92.8% | (many diffs) |
| 4 | human | 23141 | ANKLE2 | ankyrin repeat and LEM doma... | XM_006719735.1 | 72.3% | 67.9% | (many diffs) |
| 5 | human | 23141 | ANKLE2 | ankyrin repeat and LEM doma... | XM_011534787.3 | 68.1% | 67.3% | (many diffs) |
| 6 | human | 23141 | ANKLE2 | ankyrin repeat and LEM doma... | XM_011534788.1 | 68.1% | 67.3% | (many diffs) |
| 7 | human | 23141 | ANKLE2 | ankyrin repeat and LEM doma... | XR_001748638.1 | 62.1% | (many diffs) | |
| 8 | human | 23141 | ANKLE2 | ankyrin repeat and LEM doma... | XM_024448899.1 | 52.8% | 51.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2883
- ORF length:
- 2814
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gctgtggccg cggctggcgg cggccgagtg ggcggcgctg gcctgggagc 121 tgctgggcgc ctcggtgctg ctgatcgctg tgcggtggct ggtgcggcgg ctgggcccgc 181 ggccgggagg tctgggccgc agcgggaccc cagttcctcc gccaagcgcg gccgccgccc 241 ccgcctcagg tgaaatgaca atggatgctc tgttggctcg attgaaactt ctgaatccag 301 atgaccttag agaagaaatc gtcaaagccg gattgaaatg tggacccatt acatcaacta 361 caaggttcat ttttgagaaa aaattggctc aggctttact ggagcaagga ggaaggctgt 421 cttctttcta ctaccatgag gcaggtgtca cagctctcag ccaggaccca caaaggattt 481 tgaagccagc tgaagggaac ccaactgatc aggctggttt ttctgaagac agagattttg 541 gttacagtgt gggcctgaat cctccagagg aggaagctgt gacatccaag acctgctcgg 601 tgccccctag tgacaccgac acctacagag ctggagcgac tgcgtctaag gagccgcccc 661 tgtactatgg ggtgtgtcca gtgtatgagg acgtcccagc gagaaatgaa aggatctatg 721 tttatgaaaa taaaaaggaa gcattgcaag ctgtcaagat gatcaaaggg tcccgattta 781 aagctttttc taccagagaa gacgctgaga aatttgctag aggaatttgt gattatttcc 841 cttctccaag caaaacgtcc ttacaactgt ctcctgtgaa aacagctcca ctctttagca 901 atgacaggtt gaaagatggt ttgtgcttgt cggaatcaga aacagtcaac aaagagcgag 961 cgaacagtta caaaaatccc cgcacgcagg acctcaccgc caagcttcgg aaagctgtgg 1021 agaagggaga ggaggacacc ttttctgacc ttatctggag caacccccgg tatctgatag 1081 gctcaggaga caaccccact atcgtgcagg aagggtgcag gtacaacgtg atgcatgttg 1141 ctgccaaaga gaaccaggct tccatctgcc agctgactct ggacgtcctg gagaaccctg 1201 acttcatgag gctgatgtac cctgatgacg acgaggccat gctgcagaag cgtatccgtt 1261 acgtggtgga cctgtacctc aacacccccg acaagatggg ctatgacaca ccgttgcatt 1321 ttgcttgtaa gtttggaaat gcagatgtag tcaacgtgct ttcgtcacac catttgattg 1381 taaaaaactc aaggaataaa tatgataaaa cacctgaaga tgtaatttgt gaaagaagca 1441 aaaataaatc tgtggaactg aaggagcgga tcagagagta tttaaagggc cactactacg 1501 tgcccctcgt gagagcggaa gagacttctt ctccagtcat cggggagctg tggtccccag 1561 accagacggc tgaggcctct cacgtcagcc gctatggagg cagccccaga gacccggtac 1621 tgaccctgag agccttcgca gggcccctga gtccagccaa ggcagaagat tttcgcaagc 1681 tctggaaaac tccacctcga gagaaagcag gcttccttca ccacgtcaag aagtcggacc 1741 cggaaagagg ctttgagaga gtgggaaggg agctagctca tgagctgggg tatccctggg 1801 ttgaatactg ggaatttctg ggctgttttg ttgatctgtc ttcccaggaa ggcctgcaaa 1861 gactagaaga atatctcaca cagcaggaaa taggcaaaaa ggctcaacaa gaaacaggag 1921 aacgggaagc ctcctgccga gataaagcca ccacgtctgg cagcaattcc atttccgtga 1981 gggcgtttct agatgaagat gacatgaact tggaagaaat aaaaaatcgg caaaatgcag 2041 ctcgaaataa cagcccgccc acagtcggtg cttttggaca tacgaggtgc agcgccttcc 2101 ccttggagca ggaggcagac ctcatagaag ccgccgagcc gggaggtcca cacagcagca 2161 gaaatgggct ctgccatcct ctgaatcaca gcaggaccct ggcgggcaag agaccaaagg 2221 ccccccatgg ggaggaagcc catctgccac ctgtctcgga tttgactgtt gagtttgata 2281 aactgaattt gcaaaatata ggacgtagcg tttccaagac accagatgaa agtacaaaaa 2341 ctaaagatca gatcctgact tcaagaatca atgcagtaga aagagacttg ttagagcctt 2401 ctcccgcaga ccaactcggg aatggccaca ggaggacaga aagtgaaatg tcagccagga 2461 tcgctaaaat gtccttgagt cccagcagcc ccaggcacga ggatcagctc gaggtcacca 2521 gggaaCCGGC CAGGCGGCTC TTCCTTTTTG GAGAGGAGCC ATCAAAACTC GATCAGGATG 2581 TTTTGGCCGC TCTTGAATGT GCAGACGTCG ACCCCCATCA GTTCCCGGCC GTGCACAGAT 2641 GGAAGAGTGC TGTCCTGTGC TACTCACCCT CGGACAGACA GAGTTGGCCC AGTCCCGCGG 2701 TGAAAGGAAG GTTCAAGTCT CAGCTGCCAG ATCTCAGTGG CCCTCACAGC TACAGTCCGG 2761 GGAGAAACAG CGTGGCTGGA AGCAACCCCG CAAAGCCAGG CCTGGGCAGT CCTGGGCGCT 2821 ACAGCCCCGT GCACGGGAGC CAGCTCCGCA GGATGGCGCG CCTGGCTGAG CTTGCCGCCC 2881 TGTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 2941 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 3001 GGCTTTATAT ATCTTGTGGA AAGGACGATA AGGCCCGTCG TGCCAAAAAA GGACGCGTTA 3061 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt