Construct: ORF TRCN0000480383
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006234.1_s317c1
- Derived from:
- ccsbBroadEn_02793
- DNA Barcode:
- TCCCTTTGCTATTTGATTCGGGCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RASD2 (23551)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480383
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23551 | RASD2 | RASD family member 2 | NM_001366725.1 | 100% | 100% | |
| 2 | human | 23551 | RASD2 | RASD family member 2 | NM_014310.4 | 100% | 100% | |
| 3 | human | 23551 | RASD2 | RASD family member 2 | XM_011530040.2 | 100% | 100% | |
| 4 | human | 23551 | RASD2 | RASD family member 2 | XM_017028702.1 | 100% | 100% | |
| 5 | human | 23551 | RASD2 | RASD family member 2 | XM_017028701.1 | 95.6% | 95.6% | 1_36del |
| 6 | mouse | 75141 | Rasd2 | RASD family, member 2 | NM_029182.1 | 88.4% | 95.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 864
- ORF length:
- 798
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat gaagactttg tccagcggga actgcacgct cagtgtgccc gccaaaaact 121 cataccgcat ggtggtgctg ggtgcctctc gggtgggcaa gagctccatc gtgtctcgct 181 tcctcaatgg ccgctttgag gaccagtaca cacccaccat cgaggacttc caccgtaagg 241 tatacaacat ccgcggcgac atgtaccagc tcgacatcct ggatacctct ggcaaccacc 301 ccttccccgc catgcgcagg ctgtccatcc tcacagggga tgtcttcatc ctggtgttca 361 gcctggataa ccgggagtcc ttcgatgagg tcaagcgcct tcagaagcag atcctggagg 421 tcaagtcctg ccTGAAGAAC AAGACCAAGG AGGCGGCGGA GCTGCCCATG GTCATCTGTG 481 GCAACAAGAA CGACCACGGC GAGCTGTGCC GCCAGGTGCC CACCACCGAG GCCGAGCTGC 541 TGGTGTCGGG CGACGAGAAC TGCGCCTACT TCGAGGTGTC GGCCAAGAAG AACACCAACG 601 TGGACGAGAT GTTCTACGTG CTCTTCAGCA TGGCCAAGCT GCCACACGAG ATGAGCCCCG 661 CCCTGCATCG CAAGATCTCC GTGCAGTACG GTGACGCCTT CCACCCCAGG CCCTTCTGCA 721 TGCGCCGCGT CAAGGAGATG GACGCCTATG GCATGGTCTC GCCCTTCGCC CGCCGCCCCA 781 GCGTCAACAG TGACCTCAAG TACATCAAGG CCAAGGTCCT TCGGGAAGGC CAGGCCCGTG 841 AGAGGGACAA GTGCACCATC CAGTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 901 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 961 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT CCCTTTGCTA 1021 TTTGATTCGG GCTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1081 att