Construct: ORF TRCN0000480385
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003320.1_s317c1
- Derived from:
- ccsbBroadEn_10114
- DNA Barcode:
- TCGCAGTTGCTGCAGCATGTGGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AGMO (392636)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480385
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 392636 | AGMO | alkylglycerol monooxygenase | NM_001004320.2 | 99.9% | 99.7% | 338C>T |
| 2 | human | 392636 | AGMO | alkylglycerol monooxygenase | XM_011515402.3 | 95.5% | 95.2% | (many diffs) |
| 3 | human | 392636 | AGMO | alkylglycerol monooxygenase | XM_006715731.3 | 94.9% | 94.6% | 338C>T;1267_1268insTTTTTT;1269_1270ins60 |
| 4 | human | 392636 | AGMO | alkylglycerol monooxygenase | XM_017012204.1 | 94.9% | 94.6% | (many diffs) |
| 5 | human | 392636 | AGMO | alkylglycerol monooxygenase | XM_006715730.1 | 94.7% | 93.2% | (many diffs) |
| 6 | human | 392636 | AGMO | alkylglycerol monooxygenase | XR_001744759.1 | 64.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1404
- ORF length:
- 1335
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaagaaccca gaagcccagc aggatgtttc agtttcccag ggatttcgca 121 tgttgtttta cacgatgaaa cccagtgaaa cttcattcca aacattagaa gaggtgcctg 181 attatgtaaa aaaggcaact ccatttttca tttctttgat gctgcttgaa cttgttgtca 241 gctggattct caaaggaaag ccaccaggtc gcctggatga tgctttaacg tcaatctcag 301 ctggtgttct gtctcgactt ccaagtctat ttttcaggag cattgaactg accagttata 361 tttatatctg ggagaactac aggctgttca atttgccttg ggattttcca tggacttggt 421 attcagcctt cttaggagtt gactttggct actactggtt ccatcgtatg gctcatgaag 481 ttaatattat gtgggccgga caccaaacac atcatagttc tgaagactat aacttatcca 541 cagcactgag acagtctgtc ctccagatat atacttcctg gattttctac tctcccctgg 601 ccctcttcat acccccttca gtatatgctg ttcatcttca attcaatctt ctttaccaat 661 tttggatcca tacagaggtc atcaataacc ttggtccttt ggaactgatt cttaatactc 721 ctagccatca tagggttcat catggcagaa atcgttattg catagacaaa aattatgctg 781 gtgttcttat tatttgggat aaaatttttg ggacatttga agcagaaaat gaaaaagttg 841 tatatggctt aacacatccc attaatacat ttgaaccaat caaagtgcag ttccatcact 901 tattttccat atggactaca ttctgggcca cacctggatt cttcaataag ttttctgtca 961 tatttaaggg accgggatgg ggtccaggta aaccaagact tggtctcagt gaagaaattc 1021 cagaggtcac cggcaaagaa gttcccttct catcaTCTTC ATCTCAGCTA TTAAAGATAT 1081 ATACAGTTGT ACAGTTTGCT CTGATGTTGG CATTTTATGA AGAGACCTTT GCAGATACAG 1141 CTGCACTGTC GCAAGTTACT CTCCTTCTGA GGGTTTGCTT CATTATCCTG ACCTTGACTT 1201 CCATTGGATT TCTTCTGGAT CAAAGACCCA AGGCAGCTAT TATGGAAACT CTCCGTTGCT 1261 TGATGTTCTT AATGCTGTAC CGATTTGGTC ACCTGAAGCC TCTTGTCCCT TCATTGTCAT 1321 CTGCTTTTGA GATTGTTTTT TCCATTTGCA TTGCTTTCTG GGGAGTTAGA AGCATGAAAC 1381 AACTCACCTC TCACCCTTGG AAATTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1441 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1501 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT CGCAGTTGCT 1561 GCAGCATGTG GACACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1621 att