Transcript: Human XM_006715731.3

PREDICTED: Homo sapiens alkylglycerol monooxygenase (AGMO), transcript variant X5, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
AGMO (392636)
Length:
5270
CDS:
171..1442

Additional Resources:

NCBI RefSeq record:
XM_006715731.3
NBCI Gene record:
AGMO (392636)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_006715731.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000143978 CCGTTGCTTGATGTTCTTAAT pLKO.1 1355 CDS 100% 13.200 10.560 N AGMO n/a
2 TRCN0000143399 CAGGGATTTCGCATGTTGTTT pLKO.1 210 CDS 100% 5.625 4.500 N AGMO n/a
3 TRCN0000145499 GCATTGAACTGACCAGTTATA pLKO.1 442 CDS 100% 13.200 9.240 N AGMO n/a
4 TRCN0000121629 GCTGTTCATCTTCAATTCAAT pLKO.1 729 CDS 100% 5.625 3.938 N AGMO n/a
5 TRCN0000122098 CCAGTGAAACTTCATTCCAAA pLKO.1 244 CDS 100% 4.950 3.465 N AGMO n/a
6 TRCN0000143008 CCCTTCATTGTCATCTGCTTT pLKO.1 1409 CDS 100% 4.950 3.465 N AGMO n/a
7 TRCN0000144356 CTATTATGGAAACTCTCCGTT pLKO.1 1339 CDS 100% 2.640 1.848 N AGMO n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006715731.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10114 pDONR223 100% 94.9% 94.6% None 338C>T;1267_1268insTTTTTT;1269_1270ins60 n/a
2 ccsbBroad304_10114 pLX_304 0% 94.9% 94.6% V5 338C>T;1267_1268insTTTTTT;1269_1270ins60 n/a
3 TRCN0000480385 TCGCAGTTGCTGCAGCATGTGGAC pLX_317 26.4% 94.9% 94.6% V5 338C>T;1267_1268insTTTTTT;1269_1270ins60 n/a
Download CSV