Construct: ORF TRCN0000480400
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014115.4_s317c1
- Derived from:
- ccsbBroadEn_14996
- DNA Barcode:
- TTCCGCAAACCAGCTCCAAAAACG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AAK1 (22848)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480400
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 22848 | AAK1 | AP2 associated kinase 1 | NM_001371577.1 | 68.7% | 68.9% | 1396_1397insATACAATAA;1400_1401insTAGTATGAAA;1404_2022del |
| 2 | human | 22848 | AAK1 | AP2 associated kinase 1 | NM_001371575.1 | 50.3% | 50.4% | 1396_1397insATACAATAA;1400_1401insTAGTATGAAA;1404_2766del |
| 3 | human | 22848 | AAK1 | AP2 associated kinase 1 | NM_014911.4 | 48.3% | 48.4% | 1396_1397insATACAATAA;1400_1401insTAGTATGAAA;1404_2883del |
| 4 | mouse | 269774 | Aak1 | AP2 associated kinase 1 | XM_017321597.1 | 49% | 50.8% | (many diffs) |
| 5 | mouse | 269774 | Aak1 | AP2 associated kinase 1 | NM_177762.6 | 48.9% | 50.7% | (many diffs) |
| 6 | mouse | 269774 | Aak1 | AP2 associated kinase 1 | XM_006506192.1 | 44.8% | 46.5% | (many diffs) |
| 7 | mouse | 269774 | Aak1 | AP2 associated kinase 1 | NM_001040106.2 | 44.8% | 46.5% | (many diffs) |
| 8 | mouse | 269774 | Aak1 | AP2 associated kinase 1 | XM_006506191.1 | 44.5% | 46.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1488
- ORF length:
- 1422
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gaagtttttc gactcccggc gagagcaggg cggctctggc ctgggctccg 121 gctccagcgg aggagggggc agcacctcgg gcctgggcag tggctacatc ggaagagtct 181 tcggcatcgg gcgacagcag gtcacagtgg acgaggtgtt ggcggaaggt ggatttgcta 241 ttgtatttct ggtgaggaca agcaatggga tgaaatgtgc cttgaaacgc atgtttgtca 301 acaatgagca tgatctccag gtgtgcaaga gagaaatcca gataatgagg gatctttcag 361 ggcacaagaa tattgtgggt tacattgatt ctagtatcaa caacgtgagt agcggtgatg 421 tatgggaagt gctcattctg atggactttt gtagaggtgg ccaggtggta aacctgatga 481 accagcgcct gcaaacaggc tttacagaga atgaagtgct ccagatattt tgtgatacct 541 gtgaagctgt tgcccgcctg catcagtgca aaactcctat tatccaccgg gacctgaagg 601 ttgaaaacat cctcttgcat gaccgaggcc actatgtcct gtgtgacttt ggaagcgcca 661 ccaacaaatt ccagaatcca caaactgagg gagtcaatgc agtagaagat gagattaaga 721 aatacacaac gctgtcctat cgagcaccag aaatggtcaa cctgtacagt ggcaaaatca 781 tcactacgaa ggcagacatt tgggctcttg gatgtttgtt gtataaatta tgctacttca 841 ctttgccatt tggggaaagt caggtggcaa tttgtgatgg aaacttcaca attcctgata 901 attctcgata ttctcaagac atgcactgcc taattaggta tatgttggaa ccagaccctg 961 acaaaaggcc ggatatttac caggtgtcct acttctcatt taagctactc aagaaagagt 1021 gcccaattcc aaatgtacag aactctccca ttcctgcaaa gcttcctgaa ccagtgaaag 1081 ccagtgaggc agctgcAAAA AAGACCCAGC CAAAGGCCAG ACTGACAGAT CCCATTCCCA 1141 CCACAGAGAC TTCAATTGCA CCCCGCCAGA GGCCTAAAGC TGGGCAGACT CAGCCGAACC 1201 CAGGAATCCT TCCCATCCAG CCAGCGCTGA CACCCCGGAA GAGGGCCACT GTTCAGCCCC 1261 CACCTCAGGC TGCAGGATCC AGCAATCAGC CTGGCCTTTT AGCCAGTGTT CCCCAACCAA 1321 AACCCCAAGC CCCACCCAGC CAGCCTCTGC CGCAAACTCA GGCCAAGCAG CCACAGGCTC 1381 CTCCCACTCC ACAGCAGACG CCTTCTACTC AGGCCCAGGG TCTGCCCGCT CAGGCCCAGG 1441 CCACACCCCA GCACCAGCAG CATACAATAA AACTTAGTAT GAAACTTCCC AACTTTCTTG 1501 TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG 1561 TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT 1621 GGAAAGGACG ATTCCGCAAA CCAGCTCCAA AAACGACGCG TTAAGTCgac aatcaacctc 1681 tggattacaa aatttgtgaa agatt