Construct: ORF TRCN0000480401
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012888.1_s317c1
- Derived from:
- ccsbBroadEn_09262
- DNA Barcode:
- TCATAAAAGATACCATCTCGTCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GFOD1 (54438)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480401
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54438 | GFOD1 | glucose-fructose oxidoreduc... | NR_038459.1 | 27.9% | 1_433del;833T>A;842_1458del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 477
- ORF length:
- 408
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttGGCACCAT GGGGGATTCC AGGAGGGATG TGCTTCATGA GCCGGCAATT GCCCTTCTTT 121 CCAGTCCCCA GACCCAGTCA AGATTACAGA TAGATGCGTT CATCGCGCGC AGGTGCTGGG 181 GAAGCCAGTG GGACATCACT GGCTACATTC ACCAACAGGA TTTTCCAACT GCAGTATTTA 241 TCGGTTTTTC AAACGAATTC AACATCTGTA TTCCTCACCC ATTTACTGAG TGGCTACAAT 301 GTGTAAGGAA ATATTCTCAG TCATGGGGAG TTAGAAAAGG TCAAAGGCAT ATAAGATATG 361 GCATGTGCTC TGAGAGAACT CATCATCTAT CCAGAACCTA CTCATCACTT TTGGAAAGAG 421 AAGAGAAACT AGCATTCTAT GGAGTACTTA CCATGTGCCA GATTTGGAGT GAGACATTGC 481 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 541 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 601 ATATATCTTG TGGAAAGGAC GATCATAAAA GATACCATCT CGTCTAACGC GTTAAGTCga 661 caatcaacct ctggattaca aaatttgtga aagatt