Construct: ORF TRCN0000480407
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009841.2_s317c1
- Derived from:
- ccsbBroadEn_05391
- DNA Barcode:
- TCAACCCCATAATAGGGTCCACGA
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC9A9 (285195)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480407
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 285195 | SLC9A9 | solute carrier family 9 mem... | NM_173653.4 | 100% | 100% | |
| 2 | human | 285195 | SLC9A9 | solute carrier family 9 mem... | XM_017006202.2 | 84.7% | 83.7% | (many diffs) |
| 3 | human | 285195 | SLC9A9 | solute carrier family 9 mem... | XM_017006203.1 | 81.3% | 80.2% | (many diffs) |
| 4 | human | 285195 | SLC9A9 | solute carrier family 9 mem... | XM_011512703.3 | 66.4% | 66.3% | 1_1delAins649 |
| 5 | human | 285195 | SLC9A9 | solute carrier family 9 mem... | XM_011512704.3 | 54.6% | 51.9% | (many diffs) |
| 6 | mouse | 331004 | Slc9a9 | solute carrier family 9 (so... | NM_177909.5 | 86.2% | 92.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2001
- ORF length:
- 1935
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gagacagtca agggttatgt cagaaaagga tgagtatcag tttcaacatc 121 agggagcggt ggagctgctt gtcttcaatt ttttgctcat ccttaccatt ttgacaatct 181 ggttatttaa aaatcatcga ttccgcttct tgcatgaaac tggaggagca atggtgtatg 241 gccttataat gggactaatt ttacgatatg ctacagcacc aactgatatt gaaagtggaa 301 ctgtctatga ctgtgtaaaa ctaactttca gtccatcaac tctgctggtt aatatcactg 361 accaagttta tgaatataaa tacaaaagag aaataagtca gcacaacatc aatcctcatc 421 aaggaaatgc tatacttgaa aagatgacat ttgatccaga aatcttcttc aatgttttac 481 tgccaccaat tatatttcat gcaggatata gtctaaagaa gagacacttt tttcaaaact 541 taggatctat tttaacgtat gccttcttgg gaactgccat ctcctgcatc gtcatagggt 601 taattatgta tggttttgtg aaggctatga tacatgctgg ccagctgaaa aatggagact 661 ttcatttcac tgactgttta ttttttggtt cactgatgtc tgctacagat ccagtgacag 721 tgctggccat tttccatgaa ctgcacgtcg accctgacct gtacacactc ttgtttggag 781 agagtgtgtt gaatgatgca gtggccatag tccttacata ttctatatcc atttacagtc 841 ccaaggagaa tccaaatgca tttgatgccg cagcattctt ccagtctgtg gggaatttcc 901 tgggaatctt cgctggctca tttgcaatgg ggtctgcgta tgccatcatc acagcactgt 961 tgaccaaatt taccaagctg tgtgagttcc cgatgctgga aaccggcctg tttttcctgc 1021 tttcttggag tgccttcctg tctgccgagg ctgccggcct aacagggata gttgctgttc 1081 tcttctgtgg agtcacacaa gcacattata cctacaacaa tctgtcttcg gattccaaaa 1141 taagaactaa acagttgttt gaatttatga actttttggc ggagaacgtc atcttctgtt 1201 acatgggcct ggcactgttc acgttccaga atcatatctt taatgctctt tttatacttg 1261 gagcctttct agcaattttt gttgccagag cctgcaacat atatcccctc tccttcctcc 1321 tgaatctagg ccgaaaacag aagatcccct ggaactttca gcacatgatg atgttttcag 1381 gtttgcgagg agcgatcgca tttgccttag ctattcggaa cacagaatct cagcccaaac 1441 aaatgatgtt taccactacg ctgctcctcg tgttcttcac tgtctgggta tttggaggag 1501 gaacaacccc catgttgact tggcttcaga tcagagttgg cgtggacctg gatgaaaatc 1561 tgaaggagga cccctcctca caacaccagg aagcaaataa cttggataaa aacatgacga 1621 aagcagagag tgctcggctc ttcagaatgt ggtatagctt tgaCCACAAG TATCTGAAAC 1681 CAATTTTAAC CCACTCTGGT CCTCCGCTGA CTACAACATT ACCTGAATGG TGTGGTCCGA 1741 TTTCCAGGCT GCTTACCAGT CCTCAAGCCT ATGGGGAACA GCTAAAAGAG GATGATGTGG 1801 AATGCATTGT AAACCAGGAT GAACTAGCCA TAAATTACCA GGAGCAAGCC TCCTCACCCT 1861 GCAGTCCTCC TGCAAGGCTA GGTCTGGACC AGAAAGCTTC ACCCCAGACG CCAGGCAAGG 1921 AAAACATTTA TGAGGGAGAC CTCGGCCTGG GAGGCTATGA ACTCAAGCTT GAGCAAACTT 1981 TGGGTCAATC CCAGTTGAAT TACCCAATTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC 2041 TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA 2101 AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATCAAC CCCATAATAG 2161 GGTCCACGAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg tgaaagatt