Construct: ORF TRCN0000480415
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006814.1_s317c1
- Derived from:
- ccsbBroadEn_16152
- DNA Barcode:
- AAGCACCTTCTTCCATCGTAACGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TCAF2 (285966)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480415
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 285966 | TCAF2 | TRPM8 channel associated fa... | NM_173678.3 | 99.8% | 99.7% | (many diffs) |
| 2 | human | 285966 | TCAF2 | TRPM8 channel associated fa... | XM_006715928.3 | 99.8% | 99.7% | (many diffs) |
| 3 | human | 285966 | TCAF2 | TRPM8 channel associated fa... | NM_001365429.1 | 99.8% | 99.6% | (many diffs) |
| 4 | human | 285966 | TCAF2 | TRPM8 channel associated fa... | NM_001365430.1 | 99.8% | 99.6% | (many diffs) |
| 5 | human | 285966 | TCAF2 | TRPM8 channel associated fa... | NM_001363538.2 | 90.9% | 91.1% | (many diffs) |
| 6 | human | 285966 | TCAF2 | TRPM8 channel associated fa... | XM_011516059.2 | 71.9% | 69% | (many diffs) |
| 7 | human | 285966 | TCAF2 | TRPM8 channel associated fa... | NM_001130026.2 | 67.5% | 64.8% | (many diffs) |
| 8 | human | 285966 | TCAF2 | TRPM8 channel associated fa... | NM_001365428.1 | 66.6% | 66.6% | 0_1ins843;879A>T;931G>A |
| 9 | human | 653691 | TCAF2P1 | TRPM8 channel associated fa... | NR_110549.1 | 65.9% | (many diffs) | |
| 10 | human | 285966 | TCAF2 | TRPM8 channel associated fa... | NM_001365427.2 | 28.8% | 25.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2604
- ORF length:
- 2535
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgaccatt gctgctgctg cgtttgaggc cctcatggat ggagtgacat 121 gctgggatgt ccccagaggc cccatcccca gtgaactcct tcttattgga gaagccgcct 181 tccccgtgat ggtgaatgac aagggccagg tgctcattgc tgcctcctcc tacggccgag 241 gccgcctcgt ggttgtgtcc catgagggct acctgtcgca tgctggcttg gctccatttc 301 tcctcaatgc agtgagctgg ctctgtccct gtcctggggc tcccgtggga gtgcatccat 361 ccctggcacc tctagtaaac atcctacagg atgctgggct tgaggcacag gtcaagccag 421 aaccaggaga gcccctaggg gtttactgta tcaatgccta caatgacacc ttgactgcaa 481 cgctgatcca gtttgtgaaa catggagggg gcttgttaat cgggggccag gcctggtact 541 gggccagcca gcacggccct gacaaggtgc tctccaggtt ccctgggaac aaggtgacaa 601 gtgtagccgg agtgtacttc actgacacct atggggacag agaccggttc aaggtctcta 661 agaaggtgcc caagatccca ctccatgtca ggtatgggga ggatgtcagg caggaccagc 721 agcagctcct ggaggggatc tcagagctgg acatcaggac agggggagtc ccctcacagc 781 tgcttgtaca tggagccctg gccttccctc tggggctgga tgcctcactc aactgcttcc 841 tggcggctgc tcactatggc cggggccggg tggtcctggc tgcccacgag tgcctgctct 901 gtgctcccaa gatggggccc ttcttgctca atgcggtgcg ctggctggcc agaggccaga 961 caggcaaagt tggggtgaac acaaatctaa aagatctgtg tcctctccta tcggagcatg 1021 gcctgcaatg cagcctggag ccccatctga acagcgactt gtgtgtctac tgctgcaagg 1081 cgtacagtga caaggaggct aagcagctgc aggagtttgt ggctgagggt ggggggctgc 1141 tgattggggg ccaggcctgg tggtgggcct cccagaaccc tggccactgc cccttggctg 1201 gcttccctgg taacatcatc ctcaactgct ttggcctcag catcctgcct cagactctca 1261 aagcaggctg cttccccgtt cccacccctg agatgagaag ctaccacttc cgcaaggcgc 1321 tctctcaatt ccaggctata ctgaaccacg agaatgggaa cttggaaaag agctgtctgg 1381 caaagttgag agttgatggt gcagccttcc tacagattcc tgcggagggg gtccctgctt 1441 acatatccct gcacaggctc ctgaggaaga tgctacgagg gtctggcctc ccagctgtga 1501 gccgggaaaa tccagttgcc agtgactcct atgaggctgc ggtgctctcc ctggccactg 1561 ggctggctca ctctggaact gactgctccc agctggccca ggggcttggc acctggacct 1621 gctcctccag tttgtacccc tcaaaacacc ccatcaccgt ggagatcaat ggaatcaacc 1681 caggcaacaa tgattgctgg gtgagtaccg ggctctacct cctggaagga caaaatgcag 1741 aagtctcact gtctgaagct gctgcctctg ctggcctgag ggtacagatt ggctgccaca 1801 ccgatgacct taccaaggcc aggaagctat ctcgagcccc catggtgact caccaatgct 1861 ggatggacag gactgagcgg tcagtctcct gcctctgggg cggcctcctc tacgtcatcg 1921 tgcccaaggg cagccaacta ggccctgtgc ctgtcactat caggggagct gtgcctgccc 1981 catactacaa gctgggtaag acatcgctgg aggagtggaa gaggcagatg caggagaacc 2041 tggctccctg gggagagctg gccacggaca acatcatcct gacagtgcca accacaaacc 2101 ttcaggccct gaaggacccc gagcctgtgc tccgcctctg ggatgagatg atgcaggctg 2161 tggccaggct ggcggctgag cccttccctt tccgccgtcc tgagaggatt gtggctGATG 2221 TGCAGATCTC AGCTGGCTGG ATGCATTCAG GATACCCCAT CATGTGCCAC CTGGAGTCTG 2281 TGAAGGAGAT CATCAATGAG ATGGACATGA GGAGCAGGGG TGTGTGGGGC CCCATCCATG 2341 AGCTGGGCCA CAACCAACAG CGGCATGGAT GGGAGTTCCC CCCACACACT ACTGAGGCCA 2401 CCTGTAACCT TTGGTCAGTC TACGTGCATG AAACAGTCCT GGGGATCCCC AGGGCTCAGG 2461 CCCACGAGGC TCTGAGCCCT CCAGAGCGAG AGAGGAGAAT CAAGGCCCAC CTGGGAAAGG 2521 GAGCCCCCCT GTGTGACTGG AATGTATGGA CAGCCCTGGA AACATATCTA CAGGTACTGA 2581 GCAGAAATTC TGGGAGAAGG GGATTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 2641 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT TGAAAGTATT 2701 TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAA GCACCTTCTT CCATCGTAAC 2761 GTACGCGTTA AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt