Construct: ORF TRCN0000480415

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006814.1_s317c1
Derived from:
ccsbBroadEn_16152
DNA Barcode:
AAGCACCTTCTTCCATCGTAACGT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TCAF2 (285966)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000480415
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 285966 TCAF2 TRPM8 channel associated fa... NM_173678.3 99.8% 99.7% (many diffs)
2 human 285966 TCAF2 TRPM8 channel associated fa... XM_006715928.3 99.8% 99.7% (many diffs)
3 human 285966 TCAF2 TRPM8 channel associated fa... NM_001365429.1 99.8% 99.6% (many diffs)
4 human 285966 TCAF2 TRPM8 channel associated fa... NM_001365430.1 99.8% 99.6% (many diffs)
5 human 285966 TCAF2 TRPM8 channel associated fa... NM_001363538.2 90.9% 91.1% (many diffs)
6 human 285966 TCAF2 TRPM8 channel associated fa... XM_011516059.2 71.9% 69% (many diffs)
7 human 285966 TCAF2 TRPM8 channel associated fa... NM_001130026.2 67.5% 64.8% (many diffs)
8 human 285966 TCAF2 TRPM8 channel associated fa... NM_001365428.1 66.6% 66.6% 0_1ins843;879A>T;931G>A
9 human 653691 TCAF2P1 TRPM8 channel associated fa... NR_110549.1 65.9% (many diffs)
10 human 285966 TCAF2 TRPM8 channel associated fa... NM_001365427.2 28.8% 25.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2604
ORF length:
2535
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggcgaccatt gctgctgctg cgtttgaggc cctcatggat ggagtgacat
121gctgggatgt ccccagaggc cccatcccca gtgaactcct tcttattgga gaagccgcct
181tccccgtgat ggtgaatgac aagggccagg tgctcattgc tgcctcctcc tacggccgag
241gccgcctcgt ggttgtgtcc catgagggct acctgtcgca tgctggcttg gctccatttc
301tcctcaatgc agtgagctgg ctctgtccct gtcctggggc tcccgtggga gtgcatccat
361ccctggcacc tctagtaaac atcctacagg atgctgggct tgaggcacag gtcaagccag
421aaccaggaga gcccctaggg gtttactgta tcaatgccta caatgacacc ttgactgcaa
481cgctgatcca gtttgtgaaa catggagggg gcttgttaat cgggggccag gcctggtact
541gggccagcca gcacggccct gacaaggtgc tctccaggtt ccctgggaac aaggtgacaa
601gtgtagccgg agtgtacttc actgacacct atggggacag agaccggttc aaggtctcta
661agaaggtgcc caagatccca ctccatgtca ggtatgggga ggatgtcagg caggaccagc
721agcagctcct ggaggggatc tcagagctgg acatcaggac agggggagtc ccctcacagc
781tgcttgtaca tggagccctg gccttccctc tggggctgga tgcctcactc aactgcttcc
841tggcggctgc tcactatggc cggggccggg tggtcctggc tgcccacgag tgcctgctct
901gtgctcccaa gatggggccc ttcttgctca atgcggtgcg ctggctggcc agaggccaga
961caggcaaagt tggggtgaac acaaatctaa aagatctgtg tcctctccta tcggagcatg
1021gcctgcaatg cagcctggag ccccatctga acagcgactt gtgtgtctac tgctgcaagg
1081cgtacagtga caaggaggct aagcagctgc aggagtttgt ggctgagggt ggggggctgc
1141tgattggggg ccaggcctgg tggtgggcct cccagaaccc tggccactgc cccttggctg
1201gcttccctgg taacatcatc ctcaactgct ttggcctcag catcctgcct cagactctca
1261aagcaggctg cttccccgtt cccacccctg agatgagaag ctaccacttc cgcaaggcgc
1321tctctcaatt ccaggctata ctgaaccacg agaatgggaa cttggaaaag agctgtctgg
1381caaagttgag agttgatggt gcagccttcc tacagattcc tgcggagggg gtccctgctt
1441acatatccct gcacaggctc ctgaggaaga tgctacgagg gtctggcctc ccagctgtga
1501gccgggaaaa tccagttgcc agtgactcct atgaggctgc ggtgctctcc ctggccactg
1561ggctggctca ctctggaact gactgctccc agctggccca ggggcttggc acctggacct
1621gctcctccag tttgtacccc tcaaaacacc ccatcaccgt ggagatcaat ggaatcaacc
1681caggcaacaa tgattgctgg gtgagtaccg ggctctacct cctggaagga caaaatgcag
1741aagtctcact gtctgaagct gctgcctctg ctggcctgag ggtacagatt ggctgccaca
1801ccgatgacct taccaaggcc aggaagctat ctcgagcccc catggtgact caccaatgct
1861ggatggacag gactgagcgg tcagtctcct gcctctgggg cggcctcctc tacgtcatcg
1921tgcccaaggg cagccaacta ggccctgtgc ctgtcactat caggggagct gtgcctgccc
1981catactacaa gctgggtaag acatcgctgg aggagtggaa gaggcagatg caggagaacc
2041tggctccctg gggagagctg gccacggaca acatcatcct gacagtgcca accacaaacc
2101ttcaggccct gaaggacccc gagcctgtgc tccgcctctg ggatgagatg atgcaggctg
2161tggccaggct ggcggctgag cccttccctt tccgccgtcc tgagaggatt gtggctGATG
2221TGCAGATCTC AGCTGGCTGG ATGCATTCAG GATACCCCAT CATGTGCCAC CTGGAGTCTG
2281TGAAGGAGAT CATCAATGAG ATGGACATGA GGAGCAGGGG TGTGTGGGGC CCCATCCATG
2341AGCTGGGCCA CAACCAACAG CGGCATGGAT GGGAGTTCCC CCCACACACT ACTGAGGCCA
2401CCTGTAACCT TTGGTCAGTC TACGTGCATG AAACAGTCCT GGGGATCCCC AGGGCTCAGG
2461CCCACGAGGC TCTGAGCCCT CCAGAGCGAG AGAGGAGAAT CAAGGCCCAC CTGGGAAAGG
2521GAGCCCCCCT GTGTGACTGG AATGTATGGA CAGCCCTGGA AACATATCTA CAGGTACTGA
2581GCAGAAATTC TGGGAGAAGG GGATTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA
2641AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT TGAAAGTATT
2701TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAA GCACCTTCTT CCATCGTAAC
2761GTACGCGTTA AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt

Download FASTA (ORF) (Full)