Construct: ORF TRCN0000480432
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018988.3_s317c1
- Derived from:
- ccsbBroadEn_14632
- DNA Barcode:
- CTGACGCAGTTTGTCTGTGAGAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ERBB3 (2065)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480432
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2065 | ERBB3 | erb-b2 receptor tyrosine ki... | NM_001982.3 | 99.9% | 100% | 4026delG |
| 2 | human | 2065 | ERBB3 | erb-b2 receptor tyrosine ki... | NM_001005915.1 | 12.9% | 10.9% | (many diffs) |
| 3 | mouse | 13867 | Erbb3 | erb-b2 receptor tyrosine ki... | NM_010153.1 | 86.7% | 90.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 4095
- ORF length:
- 4026
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagggcgaac gacgctctgc aggtgctggg cttgcttttc agcctggccc 121 ggggctccga ggtgggcaac tctcaggcag tgtgtcctgg gactctgaat ggcctgagtg 181 tgaccggcga tgctgagaac caataccaga cactgtacaa gctctacgag aggtgtgagg 241 tggtgatggg gaaccttgag attgtgctca cgggacacaa tgccgacctc tccttcctgc 301 agtggattcg agaagtgaca ggctatgtcc tcgtggccat gaatgaattc tctactctac 361 cattgcccaa cctccgcgtg gtgcgaggga cccaggtcta cgatgggaag tttgccatct 421 tcgtcatgtt gaactataac accaactcca gccacgctct gcgccagctc cgcttgactc 481 agctcaccga gattctgtca gggggtgttt atattgagaa gaacgataag ctttgtcaca 541 tggacacaat tgactggagg gacatcgtga gggaccgaga tgctgagata gtggtgaagg 601 acaatggcag aagctgtccc ccctgtcatg aggtttgcaa ggggcgatgc tggggtcctg 661 gatcagaaga ctgccagaca ttgaccaaga ccatctgtgc tcctcagtgt aatggtcact 721 gctttgggcc caaccccaac cagtgctgcc atgatgagtg tgccgggggc tgctcaggcc 781 ctcaggacac agactgcttt gcctgccggc acttcaatga cagtggagcc tgtgtacctc 841 gctgtccaca gcctcttgtc tacaacaagc taactttcca gctggaaccc aatccccaca 901 ccaagtatca gtatggagga gtttgtgtag ccagctgtcc ccataacttt gtggtggatc 961 aaacatcctg tgtcagggcc tgtcctcctg acaagatgga agtagataaa aatgggctca 1021 agatgtgtga gccttgtggg ggactatgtc ccaaagcctg tgagggaaca ggctctggga 1081 gccgcttcca gactgtggac tcgagcaaca ttgatggatt tgtgaactgc accaagatcc 1141 tgggcaacct ggactttctg atcaccggcc tcaatggaga cccctggcac aagatccctg 1201 ccctggaccc agagaagctc aatgtcttcc ggacagtacg ggagatcaca ggttacctga 1261 acatccagtc ctggccgccc cacatgcaca acttcagtgt tttttccaat ttgacaacca 1321 ttggaggcag aagcctctac aaccggggct tctcattgtt gatcatgaag aacttgaatg 1381 tcacatctct gggcttccga tccctgaagg aaattagtgc tgggcgtatc tatataagtg 1441 ccaataggca gctctgctac caccactctt tgaactggac caaggtgctt cgggggccta 1501 cggaagagcg actagacatc aagcataatc ggccgcgcag agactgcgtg gcagagggca 1561 aagtgtgtga cccactgtgc tcctctgggg gatgctgggg cccaggccct ggtcagtgct 1621 tgtcctgtcg aaattatagc cgaggaggtg tctgtgtgac ccactgcaac tttctgaatg 1681 gggagcctcg agaatttgcc catgaggccg aatgcttctc ctgccacccg gaatgccaac 1741 ccatggaggg cactgccaca tgcaatggct cgggctctga tacttgtgct caatgtgccc 1801 attttcgaga tgggccccac tgtgtgagca gctgccccca tggagtccta ggtgccaagg 1861 gcccaatcta caagtaccca gatgttcaga atgaatgtcg gccctgccat gagaactgca 1921 cccaggggtg taaaggacca gagcttcaag actgtttagg acaaacactg gtgctgatcg 1981 gcaaaaccca tctgacaatg gctttgacag tgatagcagg attggtagtg attttcatga 2041 tgctgggcgg cacttttctc tactggcgtg ggcgccggat tcagaataaa agggctatga 2101 ggcgatactt ggaacggggt gagagcatag agcctctgga ccccagtgag aaggctaaca 2161 aagtcttggc cagaatcttc aaagagacag agctaaggaa gcttaaagtg cttggctcgg 2221 gtgtctttgg aactgtgcac aaaggagtgt ggatccctga gggtgaatca atcaagattc 2281 cagtctgcat taaagtcatt gaggacaaga gtggacggca gagttttcaa gctgtgacag 2341 atcatatgct ggccattggc agcctggacc atgcccacat tgtaaggctg ctgggactat 2401 gcccagggtc atctctgcag cttgtcactc aatatttgcc tctgggttct ctgctggatc 2461 atgtgagaca acaccggggg gcactggggc cacagctgct gctcaactgg ggagtacaaa 2521 ttgccaaggg aatgtactac cttgaggaac atggtatggt gcatagaaac ctggctgccc 2581 gaaacgtgct actcaagtca cccagtcagg ttcaggtggc agattttggt gtggctgacc 2641 tgctgcctcc tgatgataag cagctgctat acagtgaggc caagactcca attaagtgga 2701 tggcccttga gagtatccac tttgggaaat acacacacca gagtgatgtc tggagctatg 2761 gtgtgacagt ttgggagttg atgaccttcg gggcagagcc ctatgcaggg ctacgattgg 2821 ctgaagtacc agacctgcta gagaaggggg agcggttggc acagccccag atctgcacaa 2881 ttgatgtcta catggtgatg gtcaagtgtt ggatgattga tgagaacatt cgcccaacct 2941 ttaaagaact agccaatgag ttcaccagga tggcccgaga cccaccacgg tatctggtca 3001 taaagagaga gagtgggcct ggaatagccc ctgggccaga gccccatggt ctgacaaaca 3061 agaagctaga ggaagtagag ctggagccag aactagacct agacctagac ttggaagcag 3121 aggaggacaa cctggcaacc accacactgg gctccgccct cagcctacca gttggaacac 3181 ttaatcggcc acgtgggagc cagagccttt taagtccatc atctggatac atgcccatga 3241 accagggtaa tcttggggag tcttgccagg agtctgcagt ttctgggagc agtgaacggt 3301 gcccccgtcc agtctctcta cacccaatgc cacggggatg cctggcatca gagtcatcag 3361 aggggcatgt aacaggctct gaggctgagc tccaggagaa agtgtcaatg tgtaggagcc 3421 ggagcaggag ccggagccca cggccacgcg gagatagcgc ctaccattcc cagcgccaca 3481 gtctgctgac tcctgttacc ccactctccc cacccgggtt agaggaagag gatgtcaacg 3541 gttatgtcat gccagataca cacctcaaag gtactccctc ctcccgggaa ggcacccttt 3601 cttcagtggg tctcagttct gtcctgggta ctgaagaaga agatgaagat gaggagtatg 3661 aatacatgaa ccggaggaga aggcacagtc cacctcatcc ccctaggcca agttcccttg 3721 aggagctggg ttatgagtac atggatgtgg ggTCAGACCT CAGTGCCTCT CTGGGCAGCA 3781 CACAGAGTTG CCCACTCCAC CCTGTACCCA TCATGCCCAC TGCAGGCACA ACTCCAGATG 3841 AAGACTATGA ATATATGAAT CGGCAACGAG ATGGAGGTGG TCCTGGGGGT GATTATGCAG 3901 CCATGGGGGC CTGCCCAGCA TCTGAGCAAG GGTATGAAGA GATGAGAGCT TTTCAGGGGC 3961 CTGGACATCA GGCCCCCCAT GTCCATTATG CCCGCCTAAA AACTCTACGT AGCTTAGAGG 4021 CTACAGACTC TGCCTTTGAT AACCCTGATT ACTGGCATAG CAGGCTTTTC CCCAAGGCTA 4081 ATGCCCAGAG AACGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 4141 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 4201 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACTG ACGCAGTTTG TCTGTGAGAG 4261 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t