Construct: ORF TRCN0000480444
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009899.1_s317c1
- Derived from:
- ccsbBroadEn_04078
- DNA Barcode:
- CGATACCACCAGCCGGTTCTATGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GCC1 (79571)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480444
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79571 | GCC1 | GRIP and coiled-coil domain... | NM_024523.6 | 100% | 100% | |
| 2 | mouse | 74375 | Gcc1 | golgi coiled coil 1 | NM_028900.4 | 88% | 93.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2391
- ORF length:
- 2325
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gaagtttggg atgaatttcg ggggcggccc gagcaagaag gacttgctgg 121 agactataga gacccagaag aagcagcttc tccagtacca ggcacggctc aaggatgtgg 181 tccgtgccta taaaagcctg ctgaaggaga aagaggcatt agaggccagc atcaaggtgc 241 tgtcggtatc ccacgaggca gatgtgggcc tcgcaggtgt ccagcttcca ggcctcacct 301 ttcctgactc tgtggatgac cggtgctcca ctcacagcga ggatagcact gggaccgcca 361 ctagcttgga tactgcggcc agtctcacca gcaccaaggg tgagtttggg gtagaagatg 421 acagaccggc ccgtggacca ccacctccaa agtccgaaga ggccagttgg tccgagagtg 481 gcgttagcag tagcagtggg gatgggccat ttgcaggtgg ggaggtggac aaaagactgc 541 accagctgaa gactcagttg gctactttga ccagttcttt ggctacagtc actcaggaga 601 agtcccgcat ggaggcttct tacttggctg acaagaaaaa gatgaaacag gacttagagg 661 atgccagtaa caaggcggag gaggagaggg cccgcctgga gggagaattg aaggggctgc 721 aggagcaaat agcagaaacc aaagcccggc ttatcacgca gcagcatgat cgggcccaag 781 agcagagtga ccatgccttg atgctgcgtg agctccagaa gctgctgcag gaggagagga 841 cccagcgcca ggacttggag cttaggttag aagagacccg agaagccttg gcaggacgag 901 catatgcagc tgaacagatg gaaggatttg aactgcagac caagcagctg acccgtgagg 961 tggaggagct gaaaagtgaa ctgcaggcca ttcgagatga gaagaatcag ccagatcccc 1021 ggctgcaaga acttcaggaa gaggctgccc gccttaagag ccatttccag gctcagttac 1081 agcaggaaat gagaaagaca gctcttgcag aggatcaact ccgtcagcaa tctcaggtag 1141 aagaacagag ggtggcagcc ctggagaatc aaatatccga ggtgtctgag ctgctaggca 1201 cctacgagaa agccaagcag aaggaccagc tggccattca gaagctgaag gagcgcattc 1261 tgcagctgga cctggagaac aagacactgg ctctagcagc ctccagcagg tcccctttag 1321 acagccatgg agaggagtcc agtctggatg tcaatgtcct gaaagataag atggagaagc 1381 tgaagaggct gctgcaggtt gcggccagga aaagccaggt gaccctggat gtggagaagc 1441 tctgtgacct ggagataatg cccagctcgg aggctgctga tggggagaag gctactgcac 1501 tctattacca acaggagctg aaacagctga aggaagagtt tgagaggtac aagatgagag 1561 cccaggttgt cctcaaaagc aagaatacca aagatggtaa cctgggaaag gagctggagg 1621 cagcccagga acagcttgca gagctgaagg agaagtatat ttccctgcgg ctctcctgcg 1681 aggagctgga gcaccaacac cagcaggagg ctgatgactg gaagcaggag ctggcccggc 1741 tgcagcagct ccaccggcag gagctggagc ggtgccagct ggacttcagg gaccgcacac 1801 tgaaactgga ggaggagctg cacaagcagc gggatcgtgc cctagctgtg ctcaccgaga 1861 aggacttgga actggagcaa ctgcgttctg tggccttggc ctctgggctg ccaggacgca 1921 gaagtcctgt gggtggtggc ggtcctgggg acccagctga cacatcatcc tctgatagcc 1981 TGACCCAAGC ATTACAACTT GCAGCGGCCA ATGAGCCCAC TTTCTTTCTG TACGCTGAGC 2041 AACTGGCCCG CAAGGAGGTG GAGATCACAT CACTGAGGAA GCAGAAGCAC AGGCTGGAGG 2101 TCGAGGTGCA TCAGCTGCAG GATCGGCTGC TGGAGGAGGG CGAACGGCAT CGTGAGGAGG 2161 TTGCAGCCCT GCAGAGCCAC ATCGAAAAGA ACATCAGGGA CCAGAGCAGG GAGGGAGCCA 2221 ATCTGGAGTA CCTCAAAAAC ATCATCTACC GCTTCCTGAC CTTACCTGAC TCCCTGGGCC 2281 GCCAGCAGAC TCTCACAGCC ATACTGACTA TCTTGCACTT CAGTCCAGAG GAGAAACAAG 2341 TGATAATGCG ACTCCCAACC AGTGCCAGCT GGTGGCCTTC TGGCAAGAGA TGCCCAACTT 2401 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 2461 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2521 CTTGTGGAAA GGACGACGAT ACCACCAGCC GGTTCTATGT ACGCGTTAAG TCgacaatca 2581 acctctggat tacaaaattt gtgaaagatt