Construct: ORF TRCN0000480458
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017165.1_s317c1
- Derived from:
- ccsbBroadEn_09263
- DNA Barcode:
- TTAGCATACGCAGACGGGTGTTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC45A3 (85414)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480458
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 85414 | SLC45A3 | solute carrier family 45 me... | NM_033102.3 | 99.9% | 100% | 927G>T |
| 2 | mouse | 212980 | Slc45a3 | solute carrier family 45, m... | NM_001177628.2 | 87.4% | 90.7% | (many diffs) |
| 3 | mouse | 212980 | Slc45a3 | solute carrier family 45, m... | NM_145977.2 | 87.4% | 90.7% | (many diffs) |
| 4 | mouse | 212980 | Slc45a3 | solute carrier family 45, m... | XM_006529341.2 | 87.4% | 90.7% | (many diffs) |
| 5 | mouse | 212980 | Slc45a3 | solute carrier family 45, m... | XM_006529342.2 | 87.4% | 90.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1728
- ORF length:
- 1659
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggtccagagg ctgtgggtga gccgcctgct gcggcaccgg aaagcccagc 121 tcttgctggt caacctgcta acctttggcc tggaggtgtg tttggccgca ggcatcacct 181 atgtgccgcc tctgctgctg gaagtggggg tagaggagaa gttcatgacc atggtgctgg 241 gcattggtcc agtgctgggc ctggtctgtg tcccgctcct aggctcagcc agtgaccact 301 ggcgtggacg ctatggccgc cgccggccct tcatctgggc actgtccttg ggcatcctgc 361 tgagcctctt tctcatccca agggccggct ggctagcagg gctgctgtgc ccggatccca 421 ggcccctgga gctggcactg ctcatcctgg gcgtggggct gctggacttc tgtggccagg 481 tgtgcttcac tccactggag gccctgctct ctgacctctt ccgggacccg gaccactgtc 541 gccaggccta ctctgtctat gccttcatga tcagtcttgg gggctgcctg ggctacctcc 601 tgcctgccat tgactgggac accagtgccc tggcccccta cctgggcacc caggaggagt 661 gcctctttgg cctgctcacc ctcatcttcc tcacctgcgt agcagccaca ctgctggtgg 721 ctgaggaggc agcgctgggc cccaccgagc cagcagaagg gctgtcggcc ccctccttgt 781 cgccccactg ctgtccatgc cgggcccgct tggctttccg gaacctgggc gccctgcttc 841 cccggctgca ccagctgtgc tgccgcatgc cccgcaccct gcgccggctc ttcgtggctg 901 agctgtgcag ctggatggca ctcatgacct tcacgctgtt ttacacggat ttcgtgggcg 961 aggggctgta ccagggcgtg cccagagctg agcctggcac cgaggcccgg agacactatg 1021 atgaaggcgt tcggatgggc agcctggggc tgttcctgca gtgcgccatc tccctggtct 1081 tctctctggt catggaccgg ctggtgcagc gattcggcac tcgagcagtc tatttggcca 1141 gtgtggcagc tttccctgtg gctgccggtg ccacatgcct gtcccacagt gtggccgtgg 1201 tgacagcttc agccgccctc accgggttca ccttctcagc cctgcagatc ctgccctaca 1261 cactggcctc cctctaccac cgggagaagc aggtgttcct gcccaaatac cgaggggaca 1321 ctggaggtgc tagcagtgag gacagcctga tgaccagctt cctgccaggc cctaagcctg 1381 gagctccctt cccTAATGGA CACGTGGGTG CTGGAGGCAG TGGCCTGCTC CCACCTCCAC 1441 CCGCGCTCTG CGGGGCCTCT GCCTGTGATG TCTCCGTACG TGTGGTGGTG GGTGAGCCCA 1501 CCGAGGCCAG GGTGGTTCCG GGCCGGGGCA TCTGCCTGGA CCTCGCCATC CTGGATAGTG 1561 CCTTCCTGCT GTCCCAGGTG GCCCCATCCC TGTTTATGGG CTCCATTGTC CAGCTCAGCC 1621 AGTCTGTCAC TGCCTATATG GTGTCTGCCG CAGGCCTGGG TCTGGTCGCC ATTTACTTTG 1681 CTACACAGGT AGTATTTGAC AAGAGCGACT TGGCCAAATA CTCAGCGTTG CCAACTTTCT 1741 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1801 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1861 GTGGAAAGGA CGATTAGCAT ACGCAGACGG GTGTTGCACG CGTTAAGTCg acaatcaacc 1921 tctggattac aaaatttgtg aaagatt