Construct: ORF TRCN0000480462
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014907.2_s317c1
- Derived from:
- ccsbBroadEn_13124
- DNA Barcode:
- GTGATGCGAAACGTCACAGCTCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LRRIQ3 (127255)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480462
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 127255 | LRRIQ3 | leucine rich repeats and IQ... | NM_001105659.2 | 35.4% | 35.4% | 1_1209del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 729
- ORF length:
- 663
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa agagtttttt gcaccacaaa gagctggtat gaaactccga acatttagtg 121 atattgacaa atattacaca gaacaaaaga agcaggaata ccataaagaa aaagtaagag 181 ttgtagccat ggcacaagtt gctcgagaaa gagttagagt agctgttaat gaacatttga 241 atcagaaaaa atatgctaca caaaaactaa ttgaagaaaa taaagagaca attcagaaca 301 gtttacgaca agtttggcaa aacagattca actaCCTTGA AAAAGCTAGA GAGAGAAAAG 361 CTCTGTTTCT AAAAGAAAAA TCCCAAAAGG CTTCAGAGCG TTTATTAGTT CAAAACTTAA 421 ATAATGAGCG CACTCTTTTG ACCAGAGGAC TACTTAAAAT TGACAGACTG GAGAAGAATG 481 AGGCTGTCCT AAAAGAGAAA AGCCTGATTG TTAAGCAAAA ACTAAAAGCA GAAAAATATA 541 GAAAGAATTT ACTTAAAGAA ATGAAAAAAG TTAGATCTCA AGAAATATAT AAAAGACATT 601 GTGAAGAAAA ATTTGTTATG GATATGATTG CCTTTGAAAA AGCCTGTGAA AGACTTCAAG 661 ATGCTAAAAC AAAAGTAGCA ATTGTGAAAA CAAATTTAGA CTTTAAAGTT CCCAATGGAC 721 TGATAAAATG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 781 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 841 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGTGATG CGAAACGTCA CAGCTCGCAC 901 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt