Construct: ORF TRCN0000480480

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003523.1_s317c1
Derived from:
ccsbBroadEn_03922
DNA Barcode:
CGACCTGAGAACGGTCAACAAGAT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SMOC1 (64093)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000480480
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 64093 SMOC1 SPARC related modular calci... NM_022137.6 100% 100%
2 human 64093 SMOC1 SPARC related modular calci... NM_001034852.3 99.7% 99.7% 1290_1292delAGT
3 human 64093 SMOC1 SPARC related modular calci... XM_005267996.1 97.5% 97.5% 525_557del
4 human 64093 SMOC1 SPARC related modular calci... XM_005267995.1 97.3% 97.3% 525_557del;1323_1325delAGT
5 mouse 64075 Smoc1 SPARC related modular calci... NM_022316.2 86.2% 91.6% (many diffs)
6 mouse 64075 Smoc1 SPARC related modular calci... NM_001146217.1 84.2% 89.4% (many diffs)
7 mouse 64075 Smoc1 SPARC related modular calci... XM_006516135.3 79.3% 80.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1368
ORF length:
1302
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgct gcccgcgcgc tgcgcccgcc tgctcacgcc ccacttgctg ctggtgttgg
121tgcagctgtc ccctgctcgc ggccaccgca ccacaggccc caggtttcta ataagtgacc
181gtgacccaca gtgcaacctc cactgctcca ggactcaacc caaacccatc tgtgcctctg
241atggcaggtc ctacgagtcc atgtgtgagt accagcgagc caagtgccga gacccgaccc
301tgggcgtggt gcatcgaggt agatgcaaag atgctggcca gagcaagtgt cgcctggagc
361gggctcaagc cctggagcaa gccaagaagc ctcaggaagc tgtgtttgtc ccagagtgtg
421gcgaggatgg ctcctttacc caggtgcagt gccatactta cactgggtac tgctggtgtg
481tcaccccgga tgggaagccc atcagtggct cttctgtgca gaataaaact cctgtatgtt
541caggttcagt caccgacaag cccttgagcc agggtaactc aggaaggaaa gatgacgggt
601ctaagccgac acccacgatg gagacccagc cggtgttcga tggagatgaa atcacagccc
661caactctatg gattaaacac ttggtgatca aggactccaa actgaacaac accaacataa
721gaaattcaga gaaagtctat tcgtgtgacc aggagaggca gagtgccctg gaagaggccc
781agcagaatcc ccgtgagggt attgtcatcc ctgaatgtgc ccctggggga ctctataagc
841cagtgcaatg ccaccagtcc actggctact gctggtgtgt gctggtggac acagggcgcc
901cgctgcctgg gacctccaca cgctacgtga tgcccagttg tgagagcgac gccagggcca
961agactacaga ggcggatgac cccttcaagg acagggagct accaggctgt ccagaaggga
1021agaaaatgga gtttatcacc agccTACTGG ATGCTCTCAC CACTGACATG GTTCAGGCCA
1081TTAACTCAGC AGCGCCCACT GGAGGTGGGA GGTTCTCAGA GCCAGACCCC AGCCACACCC
1141TGGAGGAGCG GGTAGTGCAC TGGTATTTCA GCCAGCTGGA CAGCAATAGC AGCAACGACA
1201TTAACAAGCG GGAGATGAAG CCCTTCAAGC GCTACGTGAA GAAGAAAGCC AAGCCCAAGA
1261AATGTGCCCG GCGTTTCACC GACTACTGTG ACCTGAACAA AGACAAGGTC ATTTCACTGC
1321CTGAGCTGAA GGGCTGCCTG GGTGTTAGCA AAGAAGGACG CCTCGTCTAC CCAACTTTCT
1381TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA
1441CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT
1501GTGGAAAGGA CGACGACCTG AGAACGGTCA ACAAGATACG CGTTAAGTCg acaatcaacc
1561tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)