Construct: ORF TRCN0000480509
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003834.3_s317c1
- Derived from:
- ccsbBroadEn_15110
- DNA Barcode:
- TCCCTGGCGTCGGGGTCAATTTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PBK (55872)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480509
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55872 | PBK | PDZ binding kinase | NM_001363040.2 | 99.8% | 100% | 99G>A |
| 2 | human | 55872 | PBK | PDZ binding kinase | NM_018492.4 | 99.8% | 100% | 99G>A |
| 3 | human | 55872 | PBK | PDZ binding kinase | NM_001278945.2 | 96.5% | 96.6% | 99G>A;595_627del |
| 4 | mouse | 52033 | Pbk | PDZ binding kinase | NM_023209.2 | 84.7% | 85.4% | (many diffs) |
| 5 | mouse | 52033 | Pbk | PDZ binding kinase | XM_006519257.3 | 84.7% | 85.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1032
- ORF length:
- 966
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga agggatcagt aatttcaaga caccaagcaa attatcagaa aaaaagaaat 121 ctgtattatg ttcaactcca actataaata tcccggcctc tccatttatg cagaagcttg 181 gctttggtac tggggtaaat gtgtacctaa tgaaaagatc tccaagaggt ttgtctcatt 241 ctccttgggc tgtaaaaaag attaatccta tatgtaatga tcattatcga agtgtgtatc 301 aaaagagact aatggatgaa gctaagattt tgaaaagcct tcatcatcca aacattgttg 361 gttatcgtgc ttttactgaa gccaatgatg gcagtctgtg tcttgctatg gaatatggag 421 gtgaaaagtc tctaaatgac ttaatagaag aacgatataa agccagccaa gatccttttc 481 cagcagccat aattttaaaa gttgctttga atatggcaag agggttaaag tatctgcacc 541 aagaaaagaa actgcttcat ggagacataa agtcttcaaa tgttgtaatt aaaggcgatt 601 ttgaaacaat taaaatctgt gatgtaggag tctctctacc actggatgaa aatatgactg 661 tgactgaccc TGAGGCTTGT TACATTGGCA CAGAGCCATG GAAACCCAAA GAAGCTGTGG 721 AGGAGAATGG TGTTATTACT GACAAGGCAG ACATATTTGC CTTTGGCCTT ACTTTGTGGG 781 AAATGATGAC TTTATCGATT CCACACATTA ATCTTTCAAA TGATGATGAT GATGAAGATA 841 AAACTTTTGA TGAAAGTGAT TTTGATGATG AAGCATACTA TGCAGCGTTG GGAACTAGGC 901 CACCTATTAA TATGGAAGAA CTGGATGAAT CATACCAGAA AGTAATTGAA CTCTTCTCTG 961 TATGCACTAA TGAAGACCCT AAAGATCGTC CTTCTGCTGC ACACATTGTT GAAGCTCTGG 1021 AAACAGATGT CTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1081 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1141 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATCC CTGGCGTCGG GGTCAATTTT 1201 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t