Construct: ORF TRCN0000480543
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003698.1_s317c1
- Derived from:
- ccsbBroadEn_01425
- DNA Barcode:
- GGCCCTCGGACTTACATGTGGACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPL22 (6146)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480543
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6146 | RPL22 | ribosomal protein L22 | NM_000983.4 | 100% | 100% | |
| 2 | mouse | 19934 | Rpl22 | ribosomal protein L22 | NM_009079.3 | 89% | 99.2% | (many diffs) |
| 3 | mouse | 669393 | Gm9457 | predicted gene 9457 | XM_001473319.2 | 77.4% | 81.8% | (many diffs) |
| 4 | mouse | 19934 | Rpl22 | ribosomal protein L22 | NM_001277113.1 | 74.6% | 81.4% | (many diffs) |
| 5 | mouse | 19934 | Rpl22 | ribosomal protein L22 | NM_001277114.1 | 59.7% | 61.7% | (many diffs) |
| 6 | mouse | 19934 | Rpl22 | ribosomal protein L22 | NR_102292.1 | 14.5% | (many diffs) | |
| 7 | mouse | 19934 | Rpl22 | ribosomal protein L22 | NR_102291.1 | 14.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 450
- ORF length:
- 384
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc TCCTGTGAAA AAGCTTGTGG TGAAGGGGGG CAAAAAAAAG AAGCAAGTTC 121 TGAAGTTCAC TCTTGATTGC ACCCACCCTG TAGAAGATGG AATCATGGAT GCTGCCAATT 181 TTGAGCAGTT TTTGCAAGAA AGGATCAAAG TGAACGGAAA AGCTGGGAAC CTTGGTGGAG 241 GGGTGGTGAC CATCGAAAGG AGCAAGAGCA AGATCACCGT GACATCCGAG GTGCCTTTCT 301 CCAAAAGGTA TTTGAAATAT CTCACCAAAA AATATTTGAA GAAGAATAAT CTACGTGACT 361 GGTTGCGCGT AGTTGCTAAC AGCAAAGAGA GTTACGAATT ACGTTACTTC CAGATTAACC 421 AGGACGAAGA AGAGGAGGAA GACGAGGATT ACCCAACTTT CTTGTACAAA GTGGTTGATA 481 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 541 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGGCCC 601 TCGGACTTAC ATGTGGACAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 661 tgaaagatt