Construct: ORF TRCN0000480556
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012090.1_s317c1
- Derived from:
- ccsbBroadEn_12241
- DNA Barcode:
- CCGTAGATGGCAAGCAAACCACAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BCAS4 (55653)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480556
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55653 | BCAS4 | breast carcinoma amplified ... | NM_198799.3 | 85.2% | 85.2% | 1_90del |
| 2 | human | 55653 | BCAS4 | breast carcinoma amplified ... | XM_011528887.2 | 85% | 85.2% | 1_90del;609A>G |
| 3 | human | 55653 | BCAS4 | breast carcinoma amplified ... | XM_011528886.2 | 69.1% | 55.6% | (many diffs) |
| 4 | human | 55653 | BCAS4 | breast carcinoma amplified ... | NM_017843.4 | 63.1% | 66% | 1_90del;490_586del;633_634ins73 |
| 5 | human | 55653 | BCAS4 | breast carcinoma amplified ... | NM_001010974.2 | 63% | 63% | 1_90del;354_355ins135 |
| 6 | human | 55653 | BCAS4 | breast carcinoma amplified ... | XM_017027932.1 | 50.6% | 47.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 588
- ORF length:
- 519
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gctgctcgtg gacgctgatc agccggagcc catgcgcagc ggggcgcgcg 121 agctcgcgct cttcctgacc cccgagcctg gggccgaggc gaaggaggtg gaggagacca 181 tcgagggcat gctcctcagg ctggaagagt tttgcagcct ggctgacctg atcaggagtg 241 atacttcaca gatcctggag gaaaacatcc cagtccTTAA GGCCAAACTG ACAGAAATGC 301 GTGGCATCTA TGCCAAAGTG GACCGGCTAG AGGCCTTCGT CAAGATGGTT GGACACCACG 361 TCGCCTTCCT GGAAGCAGAC GTGCTTCAGG CTGAGCGGGA CCATGGGGCC TTCCCTCAGG 421 CCCTGCGGAG GTGGCTGGGA TCCGCAGGGC TCCCCTCCTT CAGGAACAAG TCACCTGCAC 481 CGGTGCCCGT GACGTACGAG CTGCCCACAC TGTATAGGAC GGAGGACTAT TTTCCTGTGG 541 ACGCCGGGGA AGCACAGCAC CACCCCCGCA CCTGCCCTCG GCCTTTGTTG CCAACTTTCT 601 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 661 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 721 GTGGAAAGGA CGACCGTAGA TGGCAAGCAA ACCACACACG CGTTAAGTCg acaatcaacc 781 tctggattac aaaatttgtg aaagatt