Construct: ORF TRCN0000480578
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018379.1_s317c1
- Derived from:
- ccsbBroadEn_11344
- DNA Barcode:
- CCAATCTTAGACGCTTGGCGGGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAP3K13 (9175)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480578
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9175 | MAP3K13 | mitogen-activated protein k... | XM_017007458.1 | 89.3% | 89.3% | 1_234del |
| 2 | human | 9175 | MAP3K13 | mitogen-activated protein k... | XM_017007459.2 | 89.3% | 89.3% | 1_234del |
| 3 | human | 9175 | MAP3K13 | mitogen-activated protein k... | NM_001242317.2 | 86.4% | 86.4% | 1_309del |
| 4 | human | 9175 | MAP3K13 | mitogen-activated protein k... | XM_017007457.1 | 80.2% | 80.2% | 1_483del |
| 5 | human | 9175 | MAP3K13 | mitogen-activated protein k... | NM_001242314.2 | 67.9% | 67.9% | 1_930del |
| 6 | human | 9175 | MAP3K13 | mitogen-activated protein k... | NM_004721.5 | 67.9% | 67.9% | 1_930del |
| 7 | human | 9175 | MAP3K13 | mitogen-activated protein k... | XM_011513310.2 | 67.9% | 67.9% | 1_930del |
| 8 | human | 9175 | MAP3K13 | mitogen-activated protein k... | XM_017007456.1 | 67.9% | 67.9% | 1_930del |
| 9 | mouse | 71751 | Map3k13 | mitogen-activated protein k... | NM_172821.3 | 57.1% | 60.4% | (many diffs) |
| 10 | mouse | 71751 | Map3k13 | mitogen-activated protein k... | XM_006522582.3 | 57.1% | 60.4% | (many diffs) |
| 11 | mouse | 71751 | Map3k13 | mitogen-activated protein k... | XM_006522583.3 | 57.1% | 60.4% | (many diffs) |
| 12 | mouse | 71751 | Map3k13 | mitogen-activated protein k... | XM_006522584.3 | 57.1% | 60.4% | (many diffs) |
| 13 | mouse | 71751 | Map3k13 | mitogen-activated protein k... | XM_011246015.2 | 57.1% | 60.4% | (many diffs) |
| 14 | mouse | 71751 | Map3k13 | mitogen-activated protein k... | XM_011246016.2 | 57.1% | 60.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2037
- ORF length:
- 1968
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtcatttgct ggcacggtcg catggatggc gccagaggtg atacggaatg 121 aacctgtctc tgaaaaagtt gatatatggt cttttggagt ggtgctttgg gagctgctga 181 caggagagat cccttacaaa gatgtagatt cttcagccat tatctggggt gttggaagca 241 acagcctcca ccttccagtt ccttccactt gccctgatgg attcaaaatc cttatgaaac 301 agacgtggca gagtaaacct cgaaaccgac cttcttttcg gcagacactc atgcatttag 361 acattgcctc tgcagatgta cttgccaccc cacaagaaac ttacttcaag tctcaggctg 421 aatggagaga agaagtgaaa aaacattttg agaagatcaa aagtgaagga acttgtatac 481 accggttaga tgaagaactg attcgaaggc gcagagaaga gctcaggcat gcgctggata 541 ttcgtgaaca ctatgagcgg aagcttgagc gggcgaataa tttatacatg gaattgagtg 601 ccatcatgct gcagctagaa atgcgggaga aggagctcat taagcgtgag caagcagtgg 661 aaaagaagta tcctgggacc tacaaacgac accctgttcg tcctatcatc catcccaatg 721 ccatggagaa actcatgaaa aggaaaggag tgcctcacaa atctgggatg cagaccaaac 781 ggccagactt gttgagatca gaagggatcc ccaccacaga agtggctccc actgcatccc 841 ctttgtccgg aagtcccaaa atgtccactt ctagcagcaa gagccgatat cgaagcaaac 901 cacgccaccg ccgagggaat agcagaggca gccatagtga ctttgccgca atcttgaaaa 961 accagccagc ccaggaaaat tcaccccatc ccacttacct gcaccaagct caatcccaat 1021 acccttctct tcatcaccat aattctctgc agcagcaata ccagcagccc cctcctgcca 1081 tgtcccagag tcaccatccc agactcaata tgcacggaca ggacatagca acctgcgcca 1141 acaacctgag gtatttcggc ccagcagcag ccctgcggag cccactcagc aaccatgctc 1201 agagacagct gcccggctcg agccctgacc tcatctccac agccatggct gcagactgct 1261 ggagaagttc tgagcctgac aagggccaag ctggtccctg gggctgttgc caggctgacg 1321 cttatgaccc ctgccttcag tgcaggccag aacagtatgg gtccttagac ataccctctg 1381 ctgagccagt ggggaggagc cctgaccttt ccaagtcacc agcacataat cctctcttgg 1441 aaaacgccca gagttctgag aaaacggaag aaaatgaatt cagcggctgt aggtctgagt 1501 catccctcgg cacctctcat ctcggcaccc ctccagcgct acctcgaaaa acaaggcctc 1561 tgcagaagag tggagatgac tcctcagaag aggaagaagg ggaagtagat agtgaagttg 1621 aatTTCCACG AAGACAGAGG CCCCATCGCT GTATCAGCAG CTGCCAGTCA TATTCAACCT 1681 TTAGCTCTGA GAATTTCTCT GTGTCTGATG GAGAAGAGGG AAATACCAGT GACCACTCAA 1741 ACAGTCCTGA TGAGTTAGCT GATAAACTTG AAGACCGCTT GGCAGAGAAG CTAGACGACC 1801 TGCTGTCCCA GACGCCAGAG ATTCCCATTG ACATATCCTC ACACTCGGAT GGGCTCTCTG 1861 ACAAGGAGTG TGCCGTGCGC CGTGTGAAGA CTCAGATGTC TCTGGGCAAG CTGTGTGTGG 1921 AGGAACGTGG CTATGAGAAC CCCATGCAGT TTGAAGAATC GGACTGTGAC TCTTCAGATG 1981 GGGAGTGTTC TGATGCCACA GTTAGGACCA ATAAACACTA CAGCTCTGCT ACCTGGTTGC 2041 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 2101 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 2161 ATATATCTTG TGGAAAGGAC GACCAATCTT AGACGCTTGG CGGGGTACGC GTTAAGTCga 2221 caatcaacct ctggattaca aaatttgtga aagatt