Construct: ORF TRCN0000480601
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007805.1_s317c1
- Derived from:
- ccsbBroadEn_08766
- DNA Barcode:
- CTCTGTTGAGCCCCAGAGCAGGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RBAK (57786)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480601
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57786 | RBAK | RB associated KRAB zinc finger | NM_001204456.2 | 99.8% | 100% | (many diffs) |
| 2 | human | 57786 | RBAK | RB associated KRAB zinc finger | NM_021163.4 | 99.8% | 100% | (many diffs) |
| 3 | human | 100533952 | RBAK-RBAKDN | RBAK-RBAKDN readthrough | NM_001204513.3 | 12.6% | 11.4% | (many diffs) |
| 4 | human | 100289561 | LOC100289561 | uncharacterized LOC100289561 | NM_001242713.3 | 12% | 9.4% | (many diffs) |
| 5 | human | 100289561 | LOC100289561 | uncharacterized LOC100289561 | NM_001347879.2 | 12% | 9.4% | (many diffs) |
| 6 | human | 100289561 | LOC100289561 | uncharacterized LOC100289561 | NM_001347880.2 | 12% | 9.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2211
- ORF length:
- 2142
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaacacattg caggggccag tgtcattcaa agatgtggct gtggatttca 121 cccaggagga gtggcagcag ctggaccctg atgagaagat aacttacagg gatgtgatgt 181 tggagaacta tagccatcta gtttctgtgg gatatgatac caccaagcca aacgtcatca 241 ttaagttgga gcagggagag gagccgtgga taatgggagg tgaatttcca tgtcaacata 301 gtccagaagc ctggagagtt gatgacctga tagagagaat ccaagaaaac gaagacaaac 361 attcaaggca agctgcttgt atcaatagca aaaccctgac tgaagagaaa gagaatacat 421 ttagtcaaat ttacatggaa acaagccttg ttccttcaag cataatagct cataattgtg 481 tctcatgtgg aaagaattta gaatctattt cgcaattaat tagtagtgat ggaagctatg 541 ctaggacaaa acctgatgag tgtaatgaat gtgggaaaac atatcatgga gagaaaatgt 601 gtgaatttaa tcaaaatggg gatacctatt ctcacaatga agaaaatatt cttcagaaaa 661 ttagtatttt ggagaaaccc tttgaatata atgaatgcat ggaagcctta gacaatgagg 721 ctgtttttat tgctcataag agagcttaca taggggagaa gccctatgag tggaatgatt 781 ctggaccaga cttcatacag atgtcaaatt ttaatgcata tcagagatca caaatggaaa 841 tgaagccctt tgaatgcagt gaatgtggaa aatccttctg taaaaagtca aaattcatca 901 tccaccagag ggctcacaca ggagagaaac cttatgaatg taatgtatgt gggaaatcct 961 tcagccaaaa gggaaccctc actgtacatc ggagatcaca cttagaggag aagccctata 1021 aatgtaatga atgtgggaaa accttttgtc agaagttaca cctcactcaa cacctaagaa 1081 ctcattcagg agagaaaccc tacgaatgta gcgaatgtgg gaaaaccttc tgccaaaaga 1141 cacatctcac cctgcaccag aggaatcatt caggagagag gccctatcca tgtaacgaat 1201 gtgggaaatc cttctcccgc aagtctgctc tcagtgacca tcagagaact cacacaggag 1261 agaagcttta taaatgtaat gaatgtggga aatcctacta ccgaaagtct actctgatta 1321 cacatcagag aacacacaca ggagagaagc cctatcagtg tagcgagtgt gggaaattct 1381 tttctcgggt gtcatacctc actatacatt atagaagtca tttagaagag aaaccctatg 1441 aatgtaatga atgtggcaaa accttcaatt taaattcagc cttcattaga catcggaaag 1501 tacacacaga agagaaatcc catgaatgta gtgaatgtgg aaagttctct cagttgtatc 1561 tcaccgacca tcatacagct catttagaag agaaacccta tgaatgtaat gaatgtggga 1621 aaaccttcct tgtaaattca gccttcgatg ggcaccagcc acttccaaaa ggggagaaat 1681 cctatgaatg taatgtatgt ggaaagttat tcaatgagtt gtcatactat actgaacatt 1741 atagaagtca ttcagaagag aaaccttatg gatgtagcga atgtgggaaa accttttccc 1801 ataattcatc cctcttcaga catcaaagag tacacacagg cgagaaaccc tatgaatgtt 1861 acgaatgtgg aaaattcttc tctcagaaat catatctcac tatacaTCAT CGAATTCATT 1921 CAGGAGAGAA ACCCTATGAA TGTAGTAAAT GTGGAAAAGT CTTCTCTCGG ATGTCAAACC 1981 TCACTGTCCA CTACAGAAGC CATTCAGGAG AGAAACCCTA TGAATGTAAT GAATGTGGGA 2041 AAGTCTTTTC TCAGAAGTCA TACCTCACTG TACACTATAG AACTCATTCA GGAGAGAAAC 2101 CCTATGAATG TAATGAGTGT GGGAAAAAAT TCCACCACAG ATCAGCCTTC AATAGCCATC 2161 AGAGAATTCA TAGAAGAGGA AATATGAATG TACTTGATGT GGAAAATCTC TTGCCAACTT 2221 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 2281 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2341 CTTGTGGAAA GGACGACTCT GTTGAGCCCC AGAGCAGGAA ACGCGTTAAG TCgacaatca 2401 acctctggat tacaaaattt gtgaaagatt