Transcript: Human NM_001204456.2

Homo sapiens RB associated KRAB zinc finger (RBAK), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-09-28
Taxon:
Homo sapiens (human)
Gene:
RBAK (57786)
Length:
6316
CDS:
286..2430

Additional Resources:

NCBI RefSeq record:
NM_001204456.2
NBCI Gene record:
RBAK (57786)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001204456.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000236487 GTATCTCACCGACCATCATAC pLKO_005 1773 CDS 100% 10.800 15.120 N RBAK n/a
2 TRCN0000019086 CCGACCATCATACAGCTCATT pLKO.1 1781 CDS 100% 4.950 3.960 N RBAK n/a
3 TRCN0000236488 GTGTGCCTAGTGCCAATATTT pLKO_005 3362 3UTR 100% 15.000 10.500 N RBAK n/a
4 TRCN0000236489 CCTTGTTCCTTCAAGCATAAT pLKO_005 663 CDS 100% 13.200 9.240 N RBAK n/a
5 TRCN0000019084 CGGGTGTCATACCTCACTATA pLKO.1 1603 CDS 100% 13.200 9.240 N RBAK n/a
6 TRCN0000236490 TCAATGAGTTGTCATACTATA pLKO_005 1928 CDS 100% 13.200 9.240 N RBAK n/a
7 TRCN0000019087 CCTTAGACAATGAGGCTGTTT pLKO.1 923 CDS 100% 4.950 3.465 N RBAK n/a
8 TRCN0000019085 GCTGCTTGTATCAATAGCAAA pLKO.1 589 CDS 100% 4.950 3.465 N RBAK n/a
9 TRCN0000019088 GCCTTCATTAGACATCGGAAA pLKO.1 1696 CDS 100% 4.050 2.430 N RBAK n/a
10 TRCN0000239829 AGGAGAGAAACCCTATGAATG pLKO_005 2139 CDS 100% 10.800 5.400 Y Gm14393 n/a
11 TRCN0000236491 AGTTTCTGTGGGATATGATAC pLKO_005 417 CDS 100% 10.800 5.400 Y RBAK n/a
12 TRCN0000148104 GAGTGTAATGAATGTGGGAAA pLKO.1 775 CDS 100% 4.050 2.025 Y ZNF658B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001204456.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08766 pDONR223 100% 99.8% 100% None (many diffs) n/a
2 ccsbBroad304_08766 pLX_304 0% 99.8% 100% V5 (many diffs) n/a
3 TRCN0000480601 CTCTGTTGAGCCCCAGAGCAGGAA pLX_317 14.5% 99.8% 100% V5 (many diffs) n/a
4 ccsbBroadEn_13748 pDONR223 100% 8.2% 7.1% None (many diffs) n/a
5 ccsbBroad304_13748 pLX_304 0% 8.2% 7.1% V5 (many diffs) n/a
6 TRCN0000478241 ACATAAAACGACATGACCAGGGGC pLX_317 100% 8.2% 7.1% V5 (many diffs) n/a
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