Construct: ORF TRCN0000480637
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011484.1_s317c1
- Derived from:
- ccsbBroadEn_08865
- DNA Barcode:
- ACACCTCCTAATTTATCATGCCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PARP12 (64761)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480637
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64761 | PARP12 | poly(ADP-ribose) polymerase... | NM_022750.4 | 99.8% | 100% | 1014C>A;1656C>T;1896C>G |
| 2 | human | 64761 | PARP12 | poly(ADP-ribose) polymerase... | XM_011516491.2 | 76.2% | 70.6% | (many diffs) |
| 3 | human | 64761 | PARP12 | poly(ADP-ribose) polymerase... | XM_005250038.3 | 69.1% | 68% | (many diffs) |
| 4 | human | 64761 | PARP12 | poly(ADP-ribose) polymerase... | NR_130117.2 | 64.8% | (many diffs) | |
| 5 | human | 64761 | PARP12 | poly(ADP-ribose) polymerase... | XM_005250039.4 | 59.8% | 56.6% | 1014C>A;1180_1181ins142;1260_1261ins701 |
| 6 | human | 64761 | PARP12 | poly(ADP-ribose) polymerase... | XM_005250040.4 | 58.6% | 56.6% | (many diffs) |
| 7 | human | 64761 | PARP12 | poly(ADP-ribose) polymerase... | XR_927514.2 | 48.5% | (many diffs) | |
| 8 | human | 64761 | PARP12 | poly(ADP-ribose) polymerase... | XR_001744855.1 | 48.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2169
- ORF length:
- 2103
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ccaggccggc gtcgtcggtg aggtcaccca ggtgctgtgc gcggccgggg 121 gcgccctgga gttgcccgag ctgcggcgcc gcttgcggat gggcttgagc gccgacgcgc 181 tggagcggct gctgcggcag cgtgggcgct tcgtggtggc ggtgcgggcg ggcggcgcag 241 ccgcggcccc ggagcgcgtg gtgctggccg cctcgccgct gcgcctgtgt cgcgcgcacc 301 agggctccaa gccgggctgc gtggggctct gcgcgcagct ccacctctgc aggttcatgg 361 tctacggcgc ctgcaagttc ctgagagccg ggaagaactg taggaatagt cacagcttga 421 caaccgaaca caacctgagt gtgctgagaa ctcatggcgt tgaccacctg agctataatg 481 agctatgcca actcttgttt cagaacgacc cctggctttt gccagaaatt tgccaacatt 541 acaacaaagg agatggaccc cacggctctt gtgcctttca aaagcagtgc atcaagctcc 601 atatctgcca gtatttttta cagggggaat gcaagtttgg cactagctgt aagagatccc 661 atgatttctc taattctgag aatctggaaa aattggagaa gttgggtatg agctcagacc 721 tggtgagcag gctgcctacc atttatagaa atgcacatga catcaagaat aagagctctg 781 cccccagcag agtgcctcct ctttttgtcc cacaggggac ttctgaaaga aaagacagtt 841 caggttctgt gtccccaaac actcttagcc aggaggaggg tgatcagatc tgtttgtacc 901 atatccggaa aagttgtagc tttcaagata agtgccatag agttcatttc catttgccgt 961 atcgatggca attcttggat agaggcaaat gggaggattt ggacaacatg gaacttattg 1021 aagaggcata ttgcaatccc aaaatagaaa ggatcctgtg ctctgagtca gccagtacat 1081 ttcactctca ttgtctgaac tttaacgcca tgacttacgg tgctacccag gctcgccgcc 1141 tctccacggc ctcctctgtc accaaacctc cacacttcat cctcaccact gactggattt 1201 ggtactggag tgatgagttt ggttcttggc aggaatatgg aagacagggc acggtgcacc 1261 ctgtgaccac tgtcagcagt agcgacgtgg agaaggccta cctggcctac tgtacaccgg 1321 ggtctgacgg ccaggcagcc accttgaagt tccaggccgg aaagcacaac tacgagttag 1381 atttcaaagc cttcgttcag aaaaacctgg tctatggcac aactaaaaag gtttgccgca 1441 gacccaaata cgtgtctccc caggatgtga cgaccatgca aacctgcaat accaagtttc 1501 caggcccgaa gagcatccca gactattggg actcctctgc cctgccagac ccaggctttc 1561 agaagatcac ccttagttct tcctcggaag agtatcagaa ggtctggaac ctctttaacc 1621 gcacgctgcc tttctacttt gttcagaaga ttgagcgagt acagaacctg gccctctggg 1681 aagtctacca gtggcaaaaa ggacagatgc agaagcagaa tggagggaag gccgtggacg 1741 agcggcagct gttccacggc accagcgcca tttttgtgga cgccatctgc cagcagaact 1801 ttgactggcg ggtctgtggt gttcatggca cttccTACGG CAAGGGGAGC TACTTTGCCC 1861 GAGATGCTGC ATATTCCCAC CACTACAGCA AATCCGACAC GCAGACCCAC ACGATGTTCC 1921 TGGCCCGGGT GCTGGTGGGC GAGTTCGTCA GGGGCAATGC GTCCTTTGTC CGTCCGCCGG 1981 CCAAGGAGGG CTGGAGCAAC GCCTTCTATG ATAGCTGCGT GAACAGTGTG TCCGACCCCT 2041 CCATCTTTGT GATCTTTGAG AAACACCAGG TCTACCCAGA GTATGTCATC CAGTACACCA 2101 CCTCCTCCAA GCCCTCGGTC ACACCCTCCA TCCTGCTGGC CTTGGGCTCC CTGTTCAGCA 2161 GCCGACAGTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 2221 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 2281 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAACACCT CCTAATTTAT CATGCCTTAC 2341 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt