Construct: ORF TRCN0000480646
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005757.1_s317c1
- Derived from:
- ccsbBroadEn_07257
- DNA Barcode:
- GCATTTGCACTACACCAACGTAGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BCAS1 (8537)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480646
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8537 | BCAS1 | breast carcinoma amplified ... | NM_003657.3 | 99.9% | 100% | 858C>T |
| 2 | human | 8537 | BCAS1 | breast carcinoma amplified ... | NM_001366295.1 | 97.5% | 97.6% | 858C>T;1140_1141ins42 |
| 3 | human | 8537 | BCAS1 | breast carcinoma amplified ... | NM_001366296.1 | 96.1% | 96.2% | 858C>T;1350_1351ins66 |
| 4 | human | 8537 | BCAS1 | breast carcinoma amplified ... | XM_017028111.2 | 93.7% | 93.8% | 858C>T;1140_1141ins42;1308_1309ins66 |
| 5 | human | 8537 | BCAS1 | breast carcinoma amplified ... | NM_001366298.1 | 92.7% | 92.8% | 858C>T;927_1061del |
| 6 | human | 8537 | BCAS1 | breast carcinoma amplified ... | XM_011529090.3 | 92.7% | 92.8% | 858C>T;927_1061del |
| 7 | human | 8537 | BCAS1 | breast carcinoma amplified ... | XM_005260589.3 | 90.5% | 90.6% | 858C>T;927_1061del;1275_1276ins42 |
| 8 | human | 8537 | BCAS1 | breast carcinoma amplified ... | XM_005260590.3 | 89.2% | 89.3% | 858C>T;927_1061del;1485_1486ins66 |
| 9 | human | 8537 | BCAS1 | breast carcinoma amplified ... | XM_005260591.3 | 87% | 87.1% | (many diffs) |
| 10 | human | 8537 | BCAS1 | breast carcinoma amplified ... | NM_001316361.2 | 86.5% | 86.6% | 858C>T;1182_1183ins234 |
| 11 | human | 8537 | BCAS1 | breast carcinoma amplified ... | NM_001323347.1 | 86.5% | 86.6% | 858C>T;1182_1183ins234 |
| 12 | human | 8537 | BCAS1 | breast carcinoma amplified ... | NM_001366297.1 | 84.1% | 84.2% | 858C>T;1140_1141ins276 |
| 13 | human | 8537 | BCAS1 | breast carcinoma amplified ... | XM_011529091.3 | 83.8% | 83.9% | 858C>T;927_1061del;1317_1318ins168 |
| 14 | human | 8537 | BCAS1 | breast carcinoma amplified ... | XM_005260594.3 | 80.3% | 80.4% | 858C>T;927_1061del;1317_1318ins234 |
| 15 | human | 8537 | BCAS1 | breast carcinoma amplified ... | XM_005260595.3 | 78.1% | 78.2% | 858C>T;927_1061del;1275_1276ins276 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1821
- ORF length:
- 1752
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggtaaccaa atgagtgttc cccaaagagt tgaagaccaa gagaatgaac 121 cagaagcaga gacttaccag gacaacgcgt ctgctctgaa cggggttcca gtggtggtgt 181 cgacccacac agttcagcac ttagaggaag tcgacttggg aataagtgtc aagacggata 241 atgtggccac ttcttccccc gagacaacgg agataagtgc tgttgcggat gccaacggaa 301 agaatcttgg gaaagaggcc aaacccgagg caccagctgc taaatctcgt tttttcttga 361 tgctctctcg gcctgtacca ggacgtaccg gagaccaagc cgcagattca tcccttggat 421 cagtgaagct tgatgtcagc tccaataaag ctccagcgaa caaagaccca agtgagagct 481 ggacacttcc ggtggcagct ggaccggggc aggacacaga taaaacccca gggcacgccc 541 cggcccaaga caaggtcctc tctgccgcca gggatcccac gcttctccca cctgagacag 601 ggggagcagg aggagaagct ccctccaagc ccaaggactc cagctttttt gacaaattct 661 tcaagctgga caagggacag gaaaaggtgc caggtgacag ccaacaggaa gccaagaggg 721 cagagcatca agacaaggtg gatgaggttc ctggcttatc agggcagtcc gatgatgtcc 781 ctgcagggaa ggacatagtt gacggcaagg aaaaagaagg acaagaactt ggaactgcgg 841 attgctctgt ccctggggac ccagaaggac tggagactgc aaaggacgat tcccaggcag 901 cagctatagc agagaataat aattctatca tgagtttctt taaaactctg gtttcaccta 961 acaaagctga aacaaaaaag gacccagaag acacgggtgc tgaaaagtca cccaccactt 1021 cagctgacct taagtcagac aaagccaact ttacatccca ggagacccaa ggggctggca 1081 agaattccaa aggatgcaac ccatcggggc acacacagtc cgtgacaacc cctgaacctg 1141 cgaaggaagg caccaaggag aaatcaggac ccacctctct gcctctgggc aaactgtttt 1201 ggaaaaagtc agttaaagag gactcagtcc ccacaggtgc ggaggagaat gtggtgtgtg 1261 agtcaccagt agagattata aagtccaagg aagtagaatc agccttacaa acagtggacc 1321 tcaacgaagg agatgctgca cctgaaccca cagaagcgaa actcaaaaga gaagaaagca 1381 aaccaagaac ctctctgatg gcgtttctca gacaaatgtc agtgaaaggg gatggaggga 1441 tcacccactc agaagaaata aatgggaaag actccagctg ccaaacatca gactccaCAG 1501 AAAAGACTAT CACACCGCCA GAGCCTGAAC CAACAGGAGC ACCACAGAAG GGTAAAGAGG 1561 GCTCCTCGAA GGACAAGAAG TCAGCAGCCG AGATGAACAA GCAGAAGAGC AACAAGCAGG 1621 AAGCCAAAGA ACCAGCCCAG TGCACAGAGC AGGCCACGGT GGACACGAAC TCACTGCAGA 1681 ATGGGGACAA GCTCCAAAAG AGACCTGAGA AGCGGCAGCA GTCCCTTGGG GGCTTCTTTA 1741 AAGGCCTGGG ACCAAAGCGG ATGTTGGATG CTCAAGTGCA AACAGACCCA GTATCCATCG 1801 GACCAGTTGG CAAATCCAAG TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1861 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1921 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGCAT TTGCACTACA 1981 CCAACGTAGT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt