Construct: ORF TRCN0000480659
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012452.1_s317c1
- Derived from:
- ccsbBroadEn_06041
- DNA Barcode:
- AAGCCGCATAGACGGGATTCGCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CRYAA (1409)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480659
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 102724652 | CRYAA2 | crystallin alpha A2 | NM_001314050.2 | 100% | 100% | |
| 2 | human | 1409 | CRYAA | crystallin alpha A | NM_000394.4 | 99.8% | 100% | 6C>T |
| 3 | human | 102724652 | CRYAA2 | crystallin alpha A2 | NM_001320719.1 | 70.4% | 64.4% | (many diffs) |
| 4 | human | 1409 | CRYAA | crystallin alpha A | NM_001363766.1 | 70.4% | 64.4% | (many diffs) |
| 5 | mouse | 12954 | Cryaa | crystallin, alpha A | NM_001278570.1 | 88.2% | 94.7% | (many diffs) |
| 6 | mouse | 12954 | Cryaa | crystallin, alpha A | NM_013501.3 | 77.8% | 83.6% | (many diffs) |
| 7 | mouse | 12954 | Cryaa | crystallin, alpha A | NM_001278569.1 | 75.5% | 81.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 585
- ORF length:
- 519
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tgtgaccatc cagcacccct ggttcaagcg caccctgggg cccttctacc 121 ccagccggct gttcgaccag tttttcggcg agggcctttt tgagtatgac ctgctgccct 181 tcctgtcgtc caccatcagc ccctactacc gccagtccct cttccgcacc gtgctggacT 241 CCGGCATCTC TGAGGTTCGA TCCGACCGGG ACAAGTTCGT CATCTTCCTC GATGTGAAGC 301 ACTTCTCCCC GGAGGACCTC ACCGTGAAGG TGCAGGACGA CTTTGTGGAG ATCCACGGAA 361 AGCACAACGA GCGCCAGGAC GACCACGGCT ACATTTCCCG TGAGTTCCAC CGCCGCTACC 421 GCCTGCCGTC CAACGTGGAC CAGTCGGCCC TCTCTTGCTC CCTGTCTGCC GATGGCATGC 481 TGACCTTCTG TGGCCCCAAG ATCCAGACTG GCCTGGATGC CACCCACGCC GAGCGAGCCA 541 TCCCCGTGTC GCGGGAGGAG AAGCCCACCT CGGCTCCCTC GTCCTACCCA ACTTTCTTGT 601 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 661 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 721 GAAAGGACGA AAGCCGCATA GACGGGATTC GCCGACGCGT TAAGTCgaca atcaacctct 781 ggattacaaa atttgtgaaa gatt