Construct: ORF TRCN0000480661
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012322.1_s317c1
- Derived from:
- ccsbBroadEn_09256
- DNA Barcode:
- GACCCTTAATATATATCCAAGGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SHISAL1 (85352)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480661
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 85352 | SHISAL1 | shisa like 1 | NM_001099294.2 | 99.8% | 100% | 295T>C |
| 2 | human | 85352 | SHISAL1 | shisa like 1 | XM_005261790.3 | 99.8% | 100% | 295T>C |
| 3 | mouse | 72301 | 1810041L15Rik | RIKEN cDNA 1810041L15 gene | NM_001163145.1 | 86.8% | 93.5% | (many diffs) |
| 4 | mouse | 72301 | 1810041L15Rik | RIKEN cDNA 1810041L15 gene | XM_006521441.3 | 86.8% | 93.5% | (many diffs) |
| 5 | mouse | 72301 | 1810041L15Rik | RIKEN cDNA 1810041L15 gene | XM_006521442.2 | 86.8% | 93.5% | (many diffs) |
| 6 | mouse | 72301 | 1810041L15Rik | RIKEN cDNA 1810041L15 gene | XM_006521443.3 | 86.8% | 93.5% | (many diffs) |
| 7 | mouse | 72301 | 1810041L15Rik | RIKEN cDNA 1810041L15 gene | XM_006521444.3 | 86.8% | 93.5% | (many diffs) |
| 8 | mouse | 72301 | 1810041L15Rik | RIKEN cDNA 1810041L15 gene | XM_006521445.3 | 86.8% | 93.5% | (many diffs) |
| 9 | mouse | 72301 | 1810041L15Rik | RIKEN cDNA 1810041L15 gene | NM_001347543.1 | 78.9% | 85% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 666
- ORF length:
- 597
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaccagttgt ggccagcagt ccttgaacgt gctcgccgtc ctcttctcat 121 tgctgttttc tgcagtcttg tctgcacatt tccgggtctg tgaaccatac acagaccaca 181 aaggccgcta ccactttggc ttccactgcc cccggctctc ggacaacaag accttcatcc 241 tctgttGTCA CCATAACAAC ACGGTCTTCA AATACTGCTG CAACGAGACG GAGTTCCAGG 301 CGGTGATGCA GGCGAACCTC ACGGCCAGCT CCGAGGGTTA CATGCACAAC AATTACACCG 361 CCCTGTTGGG AGTGTGGATC TATGGATTTT TCGTGTTGAT GCTGCTGGTT CTGGACCTTT 421 TGTATTACTC GGCAATGAAC TACGACATCT GCAAGGTCTA CCTGGCACGG TGGGGCATCC 481 AAGGACGATG GATGAAACAG GACCCCCGGC GGTGGGGGAA CCCCGCTCGG GCCCCTCGGC 541 CGGGTCAGCG GGCCCCACAG CCGCAGCCTC CCCCAGGCCC GCTGCCACAA GCCCCACAGG 601 CCGTGCACAC ATTGCGGGGA GATGCTCACA GCCCACCGCT GATGACCTTC CAGAGTTCGT 661 CTGCCTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 721 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 781 CTTGGCTTTA TATATCTTGT GGAAAGGACG AGACCCTTAA TATATATCCA AGGTGACGCG 841 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt