Construct: ORF TRCN0000480669
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007531.1_s317c1
- Derived from:
- ccsbBroadEn_05408
- DNA Barcode:
- GGGGGCGTCATATAACAAACAATG
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TREML4 (285852)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480669
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 285852 | TREML4 | triggering receptor express... | NM_198153.3 | 100% | 100% | |
| 2 | human | 340206 | TREML3P | triggering receptor express... | NR_027256.1 | 34.9% | (many diffs) | |
| 3 | human | 285852 | TREML4 | triggering receptor express... | XR_001743356.2 | 18.4% | 1_141del;646_787del;884_3253del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 669
- ORF length:
- 600
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcctggggt ggggtccaca cctgctgctt ccacctgtgc tgctgctgct 121 cctggcctca gggtgctgtg cctgaagaac ttcacaaaca cccaggacag accctcctcc 181 tgcaatgcca gtactcaccc aagagagggc cctatcagcc caaatcctgg tgtcagcaga 241 catctccaag tcggtgtaCC TTACTTGTCA CCAGCTCCAA GCCCTGGACA GCAGTTCAGA 301 AGTCTCATTA CACAATCTGG GACAAGCCCA ATGCTGGCTT CTTCAACATC ACCATGATTC 361 AGCTGACACA GAATGACTCG GGATTCTACT GGTGTGGAAT CTACAACGCT TCCGAAAACA 421 TCATCACTGT TCTTAGAAAT ATCAGCCTGG TGGTGTCTCC AGCCCCAACC ACGTCTCCTA 481 TGTGGACTCT TCCCTGGCTC CCAACAAGCA CAGTTCTGAT CACTTCTCCA GAGGGGACCT 541 CTGGCCATCC CTCCATCAAT GGCTCTGAGA CCAGGAAATC AAGAGCCCCT GCCTGCCTTG 601 GCTCAGGTGG CCCCAGATTC CTGGTCTTGG TGCTATGTGG ACTCCTCCTG GCCAAGGGCC 661 TGATGTTGTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC 721 TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT 781 TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGGGGGCG TCATATAACA AACAATGACG 841 CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt