Construct: ORF TRCN0000480687
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018505.1_s317c1
- Derived from:
- ccsbBroadEn_15420
- DNA Barcode:
- GACTATGGGCACATCACGTTTGAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FTL (2512)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480687
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2512 | FTL | ferritin light chain | NM_000146.4 | 57.4% | 51.4% | (many diffs) |
| 2 | human | 2512 | FTL | ferritin light chain | XM_024451447.1 | 29.2% | 26% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 387
- ORF length:
- 321
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgaTC AACAAGTTTG TACAAAAAAG 61 TTGGCATGAG CTCCCAGATT CGTCAGAATT ATTCCACCGA CGTGGAGGCA GCCGTCAACA 121 GCCTGGTCAA TTTGTACCTG CAGGCCTCCT ACACCTACCT CTCTCTGGGC TTCTATTTCG 181 ACCGCGATGA TGTGGCTCTG GAAGGCGTGA GCCACTTCTT CCGCGAATTG GCCGAGGAGA 241 AGCGCGAGGG CTACGAGCGT CTCCTGAAGA TGCAAAACCA GCGTGGCGGC CGCGCTCTCT 301 TCCAGGACAT CAAGGTAACT AGTGTGTGGG TAATGGACTA CATCTCCAAG CAGGCCGTGC 361 GCGCGAGGAG CCTTGATTTG AGGGCGTACC CAACTTTCTT GTACAAAGTG GTTGATATCG 421 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 481 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGACTATGG 541 GCACATCACG TTTGAGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 601 aagatt