Construct: ORF TRCN0000480700
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010279.1_s317c1
- Derived from:
- ccsbBroadEn_08560
- DNA Barcode:
- TTGACGCTGGAACGTCCTCTGTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RAB20 (55647)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480700
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55647 | RAB20 | RAB20, member RAS oncogene ... | NM_017817.3 | 99.8% | 100% | 324T>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 768
- ORF length:
- 702
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag gaagcccgac agcaagatcg tgctcctggg ggacatgaac gtggggaaga 121 cgtcgctgct gcagcggtat atggagcggc gcttcccgga cacggtcagc acggtgggcg 181 gcgccttcta cctgaagcag tggcgctcct acaacatctc catctgggac accgcagggc 241 gggagcagtt ccacggcctg ggctccatgt actgccgggg ggcggccgcc atcatcctca 301 cctatgatgt gaatcaccgg cagagcctgg tggagctgga ggaccggttc ctgggcctga 361 cagacacagC CAGCAAAGAC TGCCTCTTCG CCATCGTGGG GAACAAAGTG GACCTCACTG 421 AGGAGGGGGC CTTGGCGGGC CAGGAGAAGG AAGAGTGCAG TCCCAATATG GACGCTGGGG 481 ACCGTGTCTC CCCAAGGGCA CCTAAGCAGG TGCAGCTGGA GGATGCGGTG GCCCTTTATA 541 AAAAGATCCT CAAGTACAAG ATGCTGGATG AGCAGGATGT GCCGGCCGCT GAGCAAATGT 601 GCTTTGAGAC CAGCGCCAAG ACCGGCTACA ATGTGGACCT CCTGTTTGAG ACCCTCTTTG 661 ACCTGGTGGT GCCAATGATC TTACAGCAGA GAGCTGAGAG GCCGTCACAC ACAGTGGATA 721 TATCCAGTCA TAAGCCACCC AAGAGGACCA GATCTGGGTG TTGTGCCTGT CCAACTTTCT 781 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 841 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 901 GTGGAAAGGA CGATTGACGC TGGAACGTCC TCTGTTCACG CGTTAAGTCg acaatcaacc 961 tctggattac aaaatttgtg aaagatt