Construct: ORF TRCN0000480703
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007320.1_s317c1
- Derived from:
- ccsbBroadEn_09927
- DNA Barcode:
- TGATTGTTCACTGCCACGGAGCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TIPRL (261726)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480703
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 261726 | TIPRL | TOR signaling pathway regul... | NM_001031800.3 | 99.8% | 99.4% | 14G>C |
| 2 | human | 261726 | TIPRL | TOR signaling pathway regul... | NM_152902.5 | 64.3% | 63.6% | (many diffs) |
| 3 | mouse | 226591 | Tiprl | TIP41, TOR signalling pathw... | NM_145513.4 | 59% | 63% | (many diffs) |
| 4 | mouse | 226591 | Tiprl | TIP41, TOR signalling pathw... | XM_006496779.3 | 51.8% | 52% | (many diffs) |
| 5 | mouse | 226591 | Tiprl | TIP41, TOR signalling pathw... | XM_006496780.3 | 45.7% | 49% | (many diffs) |
| 6 | mouse | 226591 | Tiprl | TIP41, TOR signalling pathw... | XM_011238792.2 | 45.7% | 49% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 600
- ORF length:
- 534
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat gatccacgcc ttccagagca gccaccggga tttctgcttc gggccctGGA 121 AGCTGACGGC GTCCAAGACC CACATCATGA AGTCGGCGGA TGTGGAGAAA TTAGCCGATG 181 AATTACATAT GCCATCTCTC CCTGAAATGA TGTTTGGAGA CAACGTTTTA AGAATCCAGC 241 ATGGGTCTGG CTTTGGAATT GAGTTCAATG CTACAGATGC GTTAAGATGT GTAAACAACT 301 ACCAAGGAAT GCTTAAAGTG GCCTGTGCTG AAGAGTGGCA AGAAAGCAGG ACGGAGGGTG 361 AACACTCCAA AGAGGTTATT AAACCATATG ATTGGACCTA TACAACAGAT TATAAGGGAA 421 CCTTACTTGG AGAATCTCTT AAGTTAAAGG TTGTACCTAC AACAGATCAT ATAGATACAG 481 AAAAATTGAA AGCCAGAGAA CAGATTAAGT TTTTTGAAGA AGTTCTCCTT TTTGAGGATG 541 AACTTCATGA TCATGGAGTT TCAAGCCTGA GTGTGAAGAT TCCTGGTGGC GGGCACCTGT 601 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 661 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 721 TTTATATATC TTGTGGAAAG GACGATGATT GTTCACTGCC ACGGAGCTAA CGCGTTAAGT 781 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt