Construct: ORF TRCN0000480705
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006468.1_s317c1
- Derived from:
- ccsbBroadEn_12071
- DNA Barcode:
- TGGGCCCTATCCATCAACCTCACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RSBN1 (54665)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480705
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54665 | RSBN1 | round spermatid basic prote... | XM_017001518.2 | 89.5% | 89.5% | 1_144del |
| 2 | human | 54665 | RSBN1 | round spermatid basic prote... | NM_018364.4 | 51.2% | 51.2% | 1_144del;1378_2406del |
| 3 | human | 54665 | RSBN1 | round spermatid basic prote... | NR_130896.1 | 18.2% | 1_208del;1442_6750del | |
| 4 | mouse | 229675 | Rsbn1 | rosbin, round spermatid bas... | NM_172684.2 | 48.7% | 49.8% | (many diffs) |
| 5 | mouse | 229675 | Rsbn1 | rosbin, round spermatid bas... | XM_006501402.3 | 48.7% | 49.8% | (many diffs) |
| 6 | mouse | 229675 | Rsbn1 | rosbin, round spermatid bas... | XR_375519.3 | 47.3% | (many diffs) | |
| 7 | mouse | 229675 | Rsbn1 | rosbin, round spermatid bas... | XR_001783685.1 | 23.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1299
- ORF length:
- 1233
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaagaag 61 taggcatggc tgcgcaggtc ggagcggtgc gcgtagtacg ggcggtggcg gcgcaggagg 121 agccggacaa agaggggaag gagaaacctc atgctggggt ctccccgcgg ggagttaaac 181 ggcagcgccg atctagcagt ggggggtctc aggagaagcg ggggcggccg agccaggagc 241 cccctctcgc tccccctcac cggcggcgtc gcagccgcca acatcctggg ccgctgccgc 301 caacgaatgc agccccaact gtcccaggcc ctgttgagcc tcttctcctg ccgcctccgc 361 cgccaccttc gctggcaccc gccgggcccg ctgtcgctgc ccctctcccg gccccaagca 421 cctcggccct cttcaccttc tcgcctctga cggtgagcgc ggccgggccc aagcataagg 481 gccacaagga gcggcacaag caccatcacc accgcggccc cgatggtgat cccagctcct 541 gcggaaccga tctcaagcac aaggacaagc aggaaaacgg cgagaggact ggaggggtgc 601 ctctgatcaa agcccccaag agagaaacac cagatgaaaa tggtaaaacc cagagagccg 661 atgattttgt cttgaagaaa ataaagaaga aaaagaaaaa gaaacaccga gaagacatgc 721 gaggaagacg ccttaaaatg tacaataagg aagtacaaac cgtctgtgct ggcctgaccc 781 gcatcagtaa agaaattctc acccaaggac aaataaatag cacttcagga cttaataagg 841 agtccTTCAG GTATCTGAAA GATGAACAGC TGTGCCGATT AAATTTGGGT ATGCAAGAAT 901 ATCGGGTACC CCAGGGAGTA CAAACACCTT TTATGACTCA CCAGGAACAT TCTATTCGTA 961 GAAATTTCTT AAAAACAGGT ACTAAATTTA GCAACTTTAT TCATGAGGAA CACCAGTCCA 1021 ATGGTGGTGC TCTTGTCCTT CATGCTTACA TGGATGAACT CTCATTTTTG TCTCCAATGG 1081 AGATGGAGAG ATTTTCTGAG GAGTTTCTTG CTTTGACATT CAGTGAAAAT GAGAAAAATG 1141 CTGCTTACTA TGCTTTAGCA ATAGTGCATG GAGCGGCTGC TTATCTCCCA GACTTCTTGG 1201 ACTACTTTGC TTTTAATTTC CCCAACACTC CAGTGAAAAT GGAAATTCTG GGCAAGAAAG 1261 ATATTGAAAC AACCACCATT TCAAATTTTC ACACTCAGTA CCCAACTTTC TTGTACAAAG 1321 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1381 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1441 ACGATGGGCC CTATCCATCA ACCTCACGAC GCGTTAAGTC gacaatcaac ctctggatta 1501 caaaatttgt gaaagatt