Construct: ORF TRCN0000480722
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013571.1_s317c1
- Derived from:
- ccsbBroadEn_11628
- DNA Barcode:
- GGCTCACCTTACCTAACTACGGAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MRAS (22808)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480722
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 22808 | MRAS | muscle RAS oncogene homolog | NM_001252091.1 | 100% | 100% | |
| 2 | human | 22808 | MRAS | muscle RAS oncogene homolog | NM_001252092.2 | 100% | 100% | |
| 3 | human | 22808 | MRAS | muscle RAS oncogene homolog | NM_001252093.1 | 100% | 100% | |
| 4 | human | 22808 | MRAS | muscle RAS oncogene homolog | NM_001085049.3 | 63.4% | 63.4% | 1_228del |
| 5 | human | 22808 | MRAS | muscle RAS oncogene homolog | NM_001252090.2 | 63.4% | 63.4% | 1_228del |
| 6 | human | 22808 | MRAS | muscle RAS oncogene homolog | NM_012219.4 | 63.4% | 63.4% | 1_228del |
| 7 | human | 22808 | MRAS | muscle RAS oncogene homolog | XM_005247228.1 | 63.4% | 63.4% | 1_228del |
| 8 | human | 22808 | MRAS | muscle RAS oncogene homolog | XM_017005887.2 | 63.4% | 63.4% | 1_228del |
| 9 | human | 22808 | MRAS | muscle RAS oncogene homolog | XM_024453396.1 | 63.4% | 63.4% | 1_228del |
| 10 | human | 22808 | MRAS | muscle RAS oncogene homolog | XM_005247229.2 | 44.2% | 37% | 1_228del;448_451delGTAG;510_511ins118 |
| 11 | human | 22808 | MRAS | muscle RAS oncogene homolog | XM_024453397.1 | 44.2% | 37% | 1_228del;448_451delGTAG;510_511ins118 |
| 12 | human | 22808 | MRAS | muscle RAS oncogene homolog | XM_024453398.1 | 44.2% | 37% | 1_228del;448_451delGTAG;510_511ins118 |
| 13 | mouse | 17532 | Mras | muscle and microspikes RAS | NM_008624.3 | 56.7% | 60.5% | (many diffs) |
| 14 | mouse | 17532 | Mras | muscle and microspikes RAS | XM_006510825.2 | 56.7% | 60.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 462
- ORF length:
- 396
- Sequence:
-
1 tcttcCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGCG GGAGCAATAC ATGCGCACGG GGGATGGCTT CCTCATCGTC TACTCCGTCA 121 CTGACAAGGC CAGCTTTGAG CACGTGGACC GCTTCCACCA GCTTATCCTG CGCGTCAAAG 181 ACAGGGAGTC ATTCCCGATG ATCCTCGTGG CCAACAAGGT CGATTTGATG CACTTGAGGA 241 AGATCACCAG GGAGCAAGGA AAAGAAATGG CGACCAAACA CAATATTCCG TACATAGAAA 301 CCAGTGCCAA GGACCCACCT CTCAATGTCG ACAAAGCCTT CCATGACCTC GTTAGAGTAA 361 TTAGGCAACA GATTCCGGAA AAAAGCCAGA AGAAGAAGAA GAAAACCAAA TGGCGGGGAG 421 ACCGGGCCAC AGGCACCCAC AAACTGCAAT GTGTGATCTT GTGCCCAACT TTCTTGTACA 481 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 541 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 601 AGGACGAGGC TCACCTTACC TAACTACGGA GACGCGTTAA GTCgacaatc aacctctgga 661 ttacaaaatt tgtgaaagat t