Construct: ORF TRCN0000480752
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015780.1_s317c1
- Derived from:
- ccsbBroadEn_03812
- DNA Barcode:
- TGCGCTGAATAGTTGAGGCTCACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PBXIP1 (57326)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480752
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57326 | PBXIP1 | PBX homeobox interacting pr... | NM_020524.4 | 100% | 100% | |
| 2 | human | 57326 | PBXIP1 | PBX homeobox interacting pr... | NM_001317734.2 | 96% | 96% | 0_1ins87 |
| 3 | human | 57326 | PBXIP1 | PBX homeobox interacting pr... | NM_001317735.2 | 78.7% | 78.7% | 0_1ins465 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2259
- ORF length:
- 2193
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ctcctgccca gactctgata atagctgggt gcttgctggc tccgagagcc 121 tgccagtgga gacactgggc ccggcatcca ggatggaccc agaatctgag agagccctgc 181 aggcccctca cagcccctcc aagacagatg ggaaagaatt agctgggacc atggatggag 241 aagggacgct cttccagact gaaagccctc agtctggcag cattctaaca gaggagactg 301 aggtcaaggg caccctggaa ggtgatgttt gtggtgtgga gcctcctggc ccaggagaca 361 cagtagtcca gggagacctg caggagacca ccgtggtgac aggcctggga ccagacacac 421 aggacctgga aggccagagc cctccacaga gcctgccttc aacccccaaa gcagcttgga 481 tcagggagga gggccgctgc tccagcagtg acgatgacac cgacgtggac atggagggtc 541 tgcggagacg gcggggccgg gaggccggcc cacctcagcc catggtgccc ctggctgtgg 601 agaaccaggc tgggggtgag ggtgcaggcg gggagctggg catctccctc aacatgtgcc 661 tccttggggc cctggttctg cttggcctgg gggtcctcct cttctcaggt ggcctctcag 721 agtctgagac tgggcccatg gaggaagtgg agcggcaggt cctcccagac cccgaggtgc 781 tggaagctgt gggggacagg caggatgggc taagggaaca gctgcaggcc ccagtgcctc 841 ctgacagtgt ccccagcctg caaaacatgg gtcttctgct ggacaagctg gccaaggaga 901 accaggacat ccggctgctg caggcccagc tgcaggccca aaaggaagag cttcagagcc 961 tgatgcacca gcccaaaggg ctagaggagg agaatgccca gctccggggg gctctgcagc 1021 agggcgaagc cttccagcgg gctctggagt cagagctgca gcagctgcgg gcccggctcc 1081 aggggctgga ggccgactgt gtccggggcc cagatggggt gtgcctcagt gggggtagag 1141 gcccacaggg tgacaaggcc atcagggagc aaggccccag ggagcaggag ccagaactca 1201 gcttcctgaa gcagaaggaa cagctggagg ctgaggcaca ggcattaagg caagagttag 1261 agaggcagcg acggctgctg gggtctgtac agcaggatct ggagaggagc ttgcaggatg 1321 ccagccgcgg ggacccagct catgctggct tggctgagct gggccacaga ttggcccaga 1381 aactgcaggg cctggagaac tggggccagg accctggggt ctctgccaat gcctcaaagg 1441 cctggcacca gaagtcccac ttccagaatt ctagggagtg gagtggaaag gaaaagtggt 1501 gggatgggca gagagaccgg aaggctgagc actggaaaca taagaaggaa gaatctggcc 1561 gggaaaggaa gaagaactgg ggaggtcagg aggacaggga gccagcagga aggtggaagg 1621 agggcaggcc aagggtggag gagtcgggga gcaagaagga gggcaagcga cagggcccga 1681 aggaaccccc aaggaaaagt ggtagcttcc actcctctgg agaaaagcag aagcaacctc 1741 ggtggaggga agggactaag gacagccatg accccctgcc atcctgggca gagctgttga 1801 ggcccaagta ccgggcaccc cagggctgcT CAGGTGTGGA CGAGTGTGCC CGGCAGGAGG 1861 GCCTGACTTT CTTTGGCACA GAGCTAGCCC CAGTGCGGCA ACAGGAGCTG GCCTCTCTGC 1921 TAAGAACATA CTTGGCACGG CTGCCCTGGG CTGGGCAGCT GACCAAGGAG CTACCCCTCT 1981 CACCTGCTTT CTTTGGTGAG GATGGCATCT TCCGTCATGA CCGCCTCCGC TTCCGGGATT 2041 TTGTGGATGC CCTGGAGGAC AGCTTGGAGG AGGTGGCTGT GCAACAGACA GGTGATGATG 2101 ATGAAGTAGA TGACTTTGAG GACTTCATCT TCAGCCACTT CTTTGGAGAC AAAGCACTGA 2161 AGAAGAGGTC AGGGAAGAAG GACAAGCACT CACAGAGCCC AAGAGCTGCG GGGCCCAGGG 2221 AGGGGCACAG CCATAGCCAC CACCACCACC ACCGGGGCTG CCCAACTTTC TTGTACAAAG 2281 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 2341 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 2401 ACGATGCGCT GAATAGTTGA GGCTCACAAC GCGTTAAGTC gacaatcaac ctctggatta 2461 caaaatttgt gaaagatt