Construct: ORF TRCN0000480809
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009973.1_s317c1
- Derived from:
- ccsbBroadEn_00772
- DNA Barcode:
- ATACACATAAGGATTATTCCCCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HOXA6 (3203)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480809
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3203 | HOXA6 | homeobox A6 | NM_024014.4 | 100% | 100% | |
| 2 | mouse | 15403 | Hoxa6 | homeobox A6 | NM_010454.1 | 92.8% | 96.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 765
- ORF length:
- 699
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag ttcctatttt gtgaatccca ctttccccgg gagccttccc agcggccagg 121 actccttctt gggccagctg cccctctacc aggctggcta tgacgcgctg aggcccttcc 181 cggcctcgta cggggcgtcg agtctcccgg acaagacgta cacctcacct tgtttctacc 241 aacagtccaa ctcggtcctg gcctgcaacc gggcgtccta cgagtacggg gcctcgtgtt 301 tctattctga taaggacctc agtggcgcct cgccctcggg cagtggcaag cagaggggcc 361 ccggggacta cctgcacttt tctcccgagc agcagtacaa acccgacagc agcagcgggC 421 AGGGCAAAGC ACTCCATGAC GAAGGCGCCG ACCGGAAGTA CACGAGCCCG GTTTACCCTT 481 GGATGCAGCG GATGAACTCC TGCGCGGGTG CTGTGTATGG GAGCCATGGG CGCCGAGGCC 541 GCCAGACCTA CACGCGCTAC CAGACACTGG AGCTGGAGAA GGAGTTCCAC TTCAACCGCT 601 ACCTGACACG GCGCCGCCGC ATCGAGATCG CCAACGCGCT CTGCCTCACC GAGCGCCAGA 661 TCAAGATCTG GTTCCAGAAC CGCCGCATGA AGTGGAAAAA GGAAAACAAG CTCATCAATT 721 CCACGCAGCC CAGCGGGGAG GACTCAGAGG CAAAGGCGGG CGAGTACCCA ACTTTCTTGT 781 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 841 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 901 GAAAGGACGA ATACACATAA GGATTATTCC CCCGACGCGT TAAGTCgaca atcaacctct 961 ggattacaaa atttgtgaaa gatt