Construct: ORF TRCN0000480810
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009874.1_s317c1
- Derived from:
- ccsbBroadEn_04423
- DNA Barcode:
- TCTAAAACGGAGATCCTGACATAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM220A (84792)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480810
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84792 | FAM220A | family with sequence simila... | NM_001037163.2 | 100% | 100% | |
| 2 | human | 100507415 | FAM220CP | family with sequence simila... | XR_002956924.1 | 28.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 846
- ORF length:
- 777
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagggacaga agagggcccc tcggcacctg cctggcacaa gtgcagcagg 121 ccggaggagg tgactcggac aaactatcat gcagccttaa gaaaagaatg ccggagggcc 181 cttggcctgc agatgcaccc tcctggatga ataagcctgt ggttgatgga aattcacaaa 241 gtgaggcatt atcactggaa atgagaaagg atccgagcgg ggctggcctc tggcttcaca 301 gtggcggccc agtgcttcca tatgtgagag aatcagtaag aagaaatcca gcctcagcag 361 ccactccgag cacagccgtg ggtttgttcc ctgctccaac agagtgtttt gctcgggtgt 421 ccTGCAGTGG TGTTGAAGCT CTGGGGCGGC GAGACTGGCT GGGAGGAGGG CCCAGGGCCA 481 CTGACGGCCA CAGAGGACAG TGCCCCAAAG GAGAGCCTCG GGTGTCACGA CTGCCACGCC 541 ATCAAAAAGT GCCGGAAATG GGAAGTTTTC AGGATGACCC ACCAAGTGCT TTTCCCAAGG 601 GTCTGGGCTC TGAGTTGGAA CCCGCTTGCC TGCACTCCAT CCTGTCTGCA ACGCTGCACG 661 TGTATCCCGA AGTGCTCCTG AGTGAGGAGA CAAAACGCAT TTTCCTTGAC CGTTTAAAGC 721 CCATGTTTTC AAAGCAAACA ATAGAATTCA AGAAAATGCT TAAAAGCACC TCAGATGGTC 781 TGCAGATAAC ACTGGGGTTA CTGGCTCTGC AACCTTTTGA ATTAGCAAAT ACATTATGCC 841 ATAGTTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 901 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 961 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATCTAAAACG GAGATCCTGA CATATACGCG 1021 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt