Construct: ORF TRCN0000480811
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009772.1_s317c1
- Derived from:
- ccsbBroadEn_08700
- DNA Barcode:
- AGAATCGCCTAGACTCAGCTCGTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PLAAT1 (57110)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480811
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57110 | PLAAT1 | phospholipase A and acyltra... | NM_001366112.1 | 99.8% | 99.4% | 352A>G |
| 2 | human | 57110 | PLAAT1 | phospholipase A and acyltra... | NM_020386.5 | 99.8% | 99.4% | 352A>G |
| 3 | human | 57110 | PLAAT1 | phospholipase A and acyltra... | XM_011513035.2 | 46.8% | 41.2% | (many diffs) |
| 4 | human | 57110 | PLAAT1 | phospholipase A and acyltra... | XM_011513034.1 | 46.4% | 40.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 570
- ORF length:
- 504
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gtttaatgat tgcttcagtt tgaactaccc tggcaacccc tgcccagggg 121 acttgatcga agtgttccgt cctggctatc agcactgggc cctgtacttg ggtgatggtt 181 acgttatcaa catagcacct gtagatggca ttccTGCGTC CTTTACAAGC GCCAAGTCTG 241 TATTCAGCAG TAAGGCCCTG GTGAAAATGC AGCTCTTGAA GGATGTTGTG GGAAATGACA 301 CATACAGAAT AAACAATAAA TACGATGAAA CGTACCCCCC TCTCCCTGTG GAAGAAATCA 361 TAAAGCGGTC AGAGTTTGTA ATTGGACAGG AGGTGGCCTA TAACTTACTT GTCAACGACT 421 GTGAACATTT TGTGACATTG CTTCGCTATG GAGAAGGAGT TTCAGAGCAG GCCAACCGAG 481 CGATAAGTAC CGTTGAGTTT GTGACAGCTG CTGTTGGTGT CTTCTCATTC CTGGGCTTGT 541 TTCCAAAAGG ACAAAGAGCA AAATACTATT ACCCAACTTT CTTGTACAAA GTGGTTGATA 601 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 661 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAAGAAT 721 CGCCTAGACT CAGCTCGTAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 781 tgaaagatt